Invitrogen™

pHrodo™ Deep Red E. coli BioParticles™ Conjugate for Phagocytosis

Catalog number: P35360
Invitrogen™

pHrodo™ Deep Red E. coli BioParticles™ Conjugate for Phagocytosis

Catalog number: P35360
Catalog Number
P35360
Unit Size
5 x 2 mg
Price (USD)
Price: 510.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
P353605 x 2 mg
Price: 510.00
Your Price:
-
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pHrodo Deep Red E. coli BioParticles Conjugate for Phagocytosis is a no-wash, low background, fluorogenic reagent developed for the study of phagocytosis in a live cell system. This conjugate is non-fluorescent outside the cell, but fluoresces brightly in phagosomes. The pHrodo Deep Red BioParticles conjugate makes it easier than ever to study phagocytosis and endocytosis internalization with higher certainty of results and less optimization.

pHrodo Deep Red E. coli BioParticles Conjugate features include:
• Superior signal window—minimal background at neutral pH enables maximal signal-to-noise ratio for certainty of results
• Multiplex—Cy5 channel allows for multicolor experiments
• Low pKa—eliminates non-specific signal from non-internalized cargo and only turns on in late endosome and lysosome
• Specific detection of phagocytosis and endocytosis

pHrodo Deep Red is a low-background pH sensor dye that shows no signal in neutral conditions and only fluoresces in acidic environments. This unique property enables rapid assay development and certainty of results when investigating phagocytic pathways and their regulation by drugs and/or environmental factors. pHrodo Deep Red dye enables better discrimination of internalized cargo from outside the cell because it has an approximate pKa of 5 and does not fluoresce until it enters the late endosome and lysosome. pHrodo Deep Red dye can be detected using a Cy5 fluorescent filter set and has been validated for use in a variety of applications, including flow cytometry, fluorescent microscopy,high content analysis (HCA), and high throughput screening (HTS).

Learn more about pHrodo dyes ›

Specifications

Product Type
Bioparticle Conjugate
Sterility
Non-sterile
Color
Deep Red
Emission
655 nm
Excitation
640 nm
Label or Dye
pHrodo™ Deep Red
Form
Lyophilized Powder
Quantity
5 x 2 mg
Reagent Type
Fluorogenic
Species
E. Coli
Detection Method
Fluorescence