Achieve faster staining and more accurate results without the need for wash steps or quencher dye with pH-sensitive pHrodo Red Zymosan BioParticles Conjugate for Phagocytosis. This dye-bioparticle conjugate provides faster and more accurate results than any other dye-bioparticle conjugate and has reduced signal variability and improved timing in sensitive experiments. pHrodo Red dye conjugates are non-fluorescent outside the cell, but fluoresce bright red in phagosomes, specifically detecting intracellular phagocytosis and endocytosis. The pHrodo Red Zymosan BioParticles Conjugate can also be multiplexed with a wide variety of blue, green, and far-red dyes and reporters such as GFP, Fuo-4, calcein, NucBlue, CellEvent Caspase 3/7 green, Mitosox Green, and Mitotracker Deep Red, among many others.
The fluorescence of the pHrodo Red dye dramatically increases as pH decreases from neutral to acidic, making it an ideal tool to study phagocytosis and its regulation by drugs and/or environmental factors. Its lack of fluorescence outside the cell eliminates the need for wash steps and quencher dyes.
Use the ready-made pHrodo Red Zymosan BioParticles Conjugate in imaging or flow applications, or pHrodo Red SE, the activated succinimidyl ester, for labeling microorganisms or proteins of your choice.
In addition, pHrodo dye is available in the color green, and with E. coli, zymosan, S. aureus, and dextran 10,000 MW conjugates.
For Research Use Only. Not for human or animal therapeutic or diagnostic use.