pHrodo® Red Zymosan Bioparticles® Conjugate for Phagocytosis

Description

New pH-sensitive pHrodo® Red dye conjugates give faster and more accurate results than any other phagocytosis assay. pHrodo® Red dye conjugates are non-fluorescent outside the cell, but fluoresce brightly red in phagosomes. Get faster staining and more accurate results—without the need for wash steps or quencher dye.

• Specific detection of phagocytosis and endocytosis
• Reduced signal variability and improved timing in sensitive experiments
• Multiplex with green dyes such as GFP, Fluo-4, or calcein

The fluorescence of the novel pHrodo® Red dye dramatically increases as pH decreases from neutral to acidic, making it an ideal tool to study phagocytosis and its regulation by drugs and/or environmental factors. The lack of fluorescence outside the cell eliminates the need for wash steps and quencher dyes.

Use the ready-made pHrodo® Red Zymosan BioParticles® Conjugate in imaging or flow applications, or pHrodo® Red SE, the activated succinimidyl ester, for labeling microorganisms or proteins of your choice.

In addition, pHrodo® dye is also available in green color, with E. coli, zymosan, S. aureus, and dextran 10,000 conjugates.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

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Specifications

Color:	Red
Detection Method:	Fluorescent
Excitation⁄Emission (nm):	560⁄585
For Use With (Equipment):	Attune® Acoustic Focusing Cytometer, Confocal Microscope, Floid® Cell Imaging System, Flow Cytometer, Fluorescence Microscope, High Content Instrument
Label or Dye:	pHrodo™ Red
Product Line:	pHrodo™
Product Size:	5 x 1 mg
Species:	S. cerevisiae
Shipping Condition:	Room Temperature

Contents & storage