Invitrogen™

pHrodo™ Green STP Ester

Catalog number:  P35369

Related applications:

Cell Viability, Proliferation & Function

| Labeling Chemistry

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Description

The amine-reactive pH-sensitive pHrodo® Green STP ester dye is suitable for the creation of bioconjugates to study endocytosis and phagocytosis. pHrodo® Green dramatically increases fluorescence as the pH of its surroundings become more acidic.
• Use pH-sensitive pHrodo® Green STP ester to make pH-sensitive bioconjugates of your choice
• Get faster, more accurate results than with any other endocytosis or phagocytosis assay—no need for wash steps or quenchers
• Multiplex with red fluorescent markers such as RFP, pHrodo® Red, and many others

The increase in fluorescence of pHrodo® Green as pH changes from basic to acidic correlates with the acidification of intracellular vesicles, making it an ideal tool to study endocytosis or phagocytosis and their regulation by environmental factors, drugs, or pathogens. The spectral properties of pHrodo® Green makes it useful for multi-color experiments. pHrodo® Green can be detected using most standard green fluorescent filter sets and has been validated for use on a variety of platforms, including flow cytometry, fluorescent microscopy, and high content screening (HCS). The lack of fluorescence of pHrodo® Green in a typical extracellular environment eliminates the need for wash steps or quencher dyes in the experimental workflow.

The pHrodo® Green STP ester is an amine reactive dye that can be used to make pHrodo® Green bioconjugates in aqueous buffer. The STP ester will react with primary amines on a protein, cell, or virus to create a stable conjugate that can be used in live cell assays or stored for later use.

Amine reactive pHrodo® dye is also available with red fluorescence (see pHrodo® Red SE). In addition, thiol-reactive pHrodo® Red Maleimide and Green Maleimide are a great alternative to amine-reactive dyes for antibody labeling. Thiol labeling ensures that the dye will not attach to the binding region of the antibody. pHrodo® Green and Red conjugates, for example dextran, E. coli and S. aureus, are also available in ready-to-use form.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Chemical Reactivity: Amine
Detection Method: Fluorescent
EC Extinction Coefficient: 75000
Excitation/Emission: 505⁄525
Reactive Group: STP Ester
Shipping Condition: Room Temperature
Color: Green
Label or Dye: pHrodo™ Green
Product Line: pHrodo™
Solubility: DMSO (Dimethylsulfoxide)

Contents & storage

1 vial , store ≤ -20 °C, dessicate and protected from light.