pHrodo Red Transferrin Conjugate is used for the detection of internalized transferrin in cells. Transferrin is a monomeric serum glycoprotein (∼80,000 daltons) that binds to a specific receptor on the surface of vertebrate cells and delivers up to two Fe3+ atoms via receptor-mediated endocytosis. Once iron-carrying transferrin proteins are inside endosomes, the acidic environment favors dissociation of iron from the transferrin–receptor complex. Following the release of iron, the apotransferrin is recycled to the plasma membrane, where it is released from its receptor to scavenge more iron.
pHrodo Red is weakly fluorescent outside of cells at neutral pH, but when internalized specifically through transferrin endocytosis, it becomes brightly fluorescent in the acidic endosomes. This allows the measurement of internalized transferrin, as well as the dynamic monitoring of transferrin internalization in live cells.
Features of this transferrin conjugate include:
• pHrodo Red dye conjugated directly to highly active transferrin molecule
• Excitation/emission of 560/585 nm
• Excellent photostability
• Appropriate for multiplexing with green fluorophores such as GFP, FITC, Alexa Fluor 488
pHrodo Red Transferrin Conjugate is compatible with various platforms, such as traditional fluorescence microscopy, high content screening (HCS), flow cytometery, and microplate-based fluorimetry or high throughput screening (HTS). This reagent is also compatible with various benchtop instruments such as the Evos, FLoid, Tali, and Attune instruments.
For Research Use Only. Not for use in diagnostic procedures.