Invitrogen™

pHrodo™ Antibody Labeling Kits

Catalog number: P36014
Invitrogen™

pHrodo™ Antibody Labeling Kits

Catalog number: P36014
Elevate biopharmaceutical research with pHrodo Antibody Labeling kits, designed to characterize antibody internalization, a critical step during antibody-drug conjugate research and development. Kits provide a simple, detailed protocol and advanced purification columns to easily label and purify antibodies and other large proteins. Amine-reactive pHrodo dyes covalently bind to antibodies and other proteins to produce bright, soluble conjugates whose fluorescence increases in acidic organelles including endosomes and lysosomes. Antibody internalization can be evaluated using flow cytometry, fluorescence microscopy, and high-content screening.
 
Catalog Number
P36014
Unit Size
3 labelings
Description
iFL Red Microscale Protein Labeling Kit, 3 labelings
Price (USD)
Price: 710.00
Online price: 681.65
Your price:
Availability
-
Full specifications
Quantity
DescriptioniFL Red Microscale Protein Labeling Kit, 3 labelings
For Use With (Application)Cell Analysis
Product TypeProtein Labeling Kit
Quantity1 Kit
Sufficient For3 Labels
Excitation/Emission560/585
For Use With (Equipment)Flow Cytometer
Shipping ConditionRoom Temperature
Product LinepHrodo™
ColorRed
Unit Size3 labelings
Catalog Number
Unit Size
3 x 100 µg reactions
Description
Deep Red Antibody Labeling Kit, 3 x 100 μg
Price (USD)
Price: 700.00
Online price: 665.65
Your price:
Availability
-
Full specifications
Quantity
DescriptionDeep Red Antibody Labeling Kit, 3 x 100 μg
For Use With (Application)Flow Cytometry, Fluoresence Microscopy
Product TypeLabeling Kit
Quantity3 x 100 μg Reactions
Detection MethodFluorescence
Excitation/Emission640/655 nm
Labeling Scale100 μg
Chemical ReactivityAmine
Reactive MoietyTFP Ester
SolubilityWater, DMSO (Dimethylsulfoxide)
Label or DyepHrodo Deep Red
ColorDeep Red
Labeling TargetAntibodies, Proteins
Unit Size3 x 100 µg reactions
Catalog Number
Unit Size
3 x 1 mg reactions
Description
Deep Red Antibody Labeling Kit, 3 x 1 mg
Price (USD)
Price: 720.00
Online price: 699.65
Your price:
Availability
-
Full specifications
Quantity
DescriptionDeep Red Antibody Labeling Kit, 3 x 1 mg
For Use With (Application)Flow Cytometry, Fluoresence Microscopy
Product TypeLabeling Kit
Quantity3 x 1 mg Reactions
Detection MethodFluorescence
Excitation/Emission640/655 nm
Labeling Scale1 mg
Chemical ReactivityAmine
Reactive MoietyTFP Ester
SolubilityWater
Label or DyepHrodo Deep Red
ColorDeep Red
Labeling TargetAntibodies
Unit Size3 x 1 mg reactions
Catalog Number
Unit Size
3 x 100 μg reactions
Description
Red Antibody Labeling Kit, 3 x 100 μg
Price (USD)
Price: 595.00
Your price:
Availability
-
Full specifications
Quantity
DescriptionRed Antibody Labeling Kit, 3 x 100 μg
For Use With (Application)Flow Cytometry, Fluoresence Microscopy
Product TypeLabeling Kit
Quantity3 x 100 μg Reactions
Detection MethodFluorescence
Excitation/Emission560/585 nm
Labeling Scale100 μg
Product LinepHrodo™
Chemical ReactivityAmine
Reactive MoietySTP Ester
SolubilityDMSO (Dimethylsulfoxide)
Label or DyepHrodo Red
SpacerMid-length, Pegylated
ColorRed
Labeling TargetAntibodies, Proteins
Flow Cytometer Laser Lines561 nm
Label TypepHrodo
Unit Size3 x 100 μg reactions
Catalog Number
Unit Size
3 x 1 mg reactions
Description
Red Antibody Labeling Kit, 3 x 1 mg
Price (USD)
Price: 635.00
Your price:
Availability
-
Full specifications
Quantity
DescriptionRed Antibody Labeling Kit, 3 x 1 mg
For Use With (Application)Flow Cytometry, Fluoresence Microscopy
Product TypeLabeling Kit
Quantity3 x 1 mg Reactions
Detection MethodFluorescence
Excitation/Emission560/585 nm
Labeling Scale1 mg
Product LinepHrodo™
Chemical ReactivityAmine
Reactive MoietySTP Ester
SolubilityDMSO (Dimethylsulfoxide)
Label or DyepHrodo Red
ColorRed
Labeling TargetAntibodies, Proteins
Flow Cytometer Laser Lines561 nm
Label TypepHrodo
Unit Size3 x 1 mg reactions
Catalog Number
Unit Size
3 x 100 μg reactions
Description
Green Antibody Labeling Kit, 3 x 100 μg
Price (USD)
Price: 595.00
Your price:
Availability
-
Full specifications
Quantity
DescriptionGreen Antibody Labeling Kit, 3 x 100 μg
For Use With (Application)Flow Cytometry, Fluoresence Microscopy
Product TypeLabeling Kit
Quantity3 x 100 μg Reactions
Detection MethodFluorescence
Excitation/Emission505/525 nm
Labeling Scale100 μg
Product LinepHrodo™
Chemical ReactivityAmine
Reactive MoietySTP Ester
SolubilityDMSO (Dimethylsulfoxide)
Label or DyepHrodo Green
SpacerMid-length, Pegylated
ColorGreen
Labeling TargetAntibodies, Proteins
Flow Cytometer Laser Lines488 nm
Label TypepHrodo
Unit Size3 x 100 μg reactions
Catalog Number
Unit Size
3 x 1 mg reactions
Description
Green Antibody Labeling Kit, 3 x 1 mg
Price (USD)
Price: 635.00
Your price:
Availability
-
Full specifications
Quantity
DescriptionGreen Antibody Labeling Kit, 3 x 1 mg
For Use With (Application)Flow Cytometry, Fluoresence Microscopy
Product TypeLabeling Kit
Quantity3 x 1 mg Reactions
Detection MethodFluorescence
Excitation/Emission505/525 nm
Labeling Scale1 mg
Product LinepHrodo™
Chemical ReactivityAmine
Reactive MoietySTP Ester
SolubilityDMSO (Dimethylsulfoxide)
Label or DyepHrodo Green
SpacerMid-length, Pegylated
ColorGreen
Labeling TargetAntibodies, Proteins
Flow Cytometer Laser Lines488 nm
Label TypepHrodo
Unit Size3 x 1 mg reactions
Catalog Number
Unit Size
3 labelings
Description
iFL Green Microscale Protein Labeling Kit, 3 labelings
Price (USD)
Price: 710.00
Online price: 693.65
Your price:
Availability
-
Full specifications
Quantity
DescriptioniFL Green Microscale Protein Labeling Kit, 3 labelings
For Use With (Application)Cell Analysis
Product TypeProtein Labeling Kit
Quantity1 Kit
Excitation/Emission505/525 nm
For Use With (Equipment)Flow Cytometer
Shipping ConditionRoom Temperature
Product LinepHrodo™
ColorGreen
Unit Size3 labelings
Showing 8 of 8
Catalog NumberSpecificationsUnit SizeDescriptionPrice (USD)AvailabilityQuantity
P36014Full specifications
3 labelingsiFL Red Microscale Protein Labeling Kit, 3 labelings
Price: 710.00
Online price: 681.65
Your price:
-
DescriptioniFL Red Microscale Protein Labeling Kit, 3 labelings
For Use With (Application)Cell Analysis
Product TypeProtein Labeling Kit
Quantity1 Kit
Sufficient For3 Labels
Excitation/Emission560/585
For Use With (Equipment)Flow Cytometer
Shipping ConditionRoom Temperature
Product LinepHrodo™
ColorRed
Unit Size3 labelings
P35355Full specifications
3 x 100 µg reactionsDeep Red Antibody Labeling Kit, 3 x 100 μg
Price: 700.00
Online price: 665.65
Your price:
-
DescriptionDeep Red Antibody Labeling Kit, 3 x 100 μg
For Use With (Application)Flow Cytometry, Fluoresence Microscopy
Product TypeLabeling Kit
Quantity3 x 100 μg Reactions
Detection MethodFluorescence
Excitation/Emission640/655 nm
Labeling Scale100 μg
Chemical ReactivityAmine
Reactive MoietyTFP Ester
SolubilityWater, DMSO (Dimethylsulfoxide)
Label or DyepHrodo Deep Red
ColorDeep Red
Labeling TargetAntibodies, Proteins
Unit Size3 x 100 µg reactions
P35356Full specifications
3 x 1 mg reactionsDeep Red Antibody Labeling Kit, 3 x 1 mg
Price: 720.00
Online price: 699.65
Your price:
-
DescriptionDeep Red Antibody Labeling Kit, 3 x 1 mg
For Use With (Application)Flow Cytometry, Fluoresence Microscopy
Product TypeLabeling Kit
Quantity3 x 1 mg Reactions
Detection MethodFluorescence
Excitation/Emission640/655 nm
Labeling Scale1 mg
Chemical ReactivityAmine
Reactive MoietyTFP Ester
SolubilityWater
Label or DyepHrodo Deep Red
ColorDeep Red
Labeling TargetAntibodies
Unit Size3 x 1 mg reactions
P36020Full specifications
3 x 100 μg reactionsRed Antibody Labeling Kit, 3 x 100 μg
Price: 595.00
Your price:
-
DescriptionRed Antibody Labeling Kit, 3 x 100 μg
For Use With (Application)Flow Cytometry, Fluoresence Microscopy
Product TypeLabeling Kit
Quantity3 x 100 μg Reactions
Detection MethodFluorescence
Excitation/Emission560/585 nm
Labeling Scale100 μg
Product LinepHrodo™
Chemical ReactivityAmine
Reactive MoietySTP Ester
SolubilityDMSO (Dimethylsulfoxide)
Label or DyepHrodo Red
SpacerMid-length, Pegylated
ColorRed
Labeling TargetAntibodies, Proteins
Flow Cytometer Laser Lines561 nm
Label TypepHrodo
Unit Size3 x 100 μg reactions
P36021Full specifications
3 x 1 mg reactionsRed Antibody Labeling Kit, 3 x 1 mg
Price: 635.00
Your price:
-
DescriptionRed Antibody Labeling Kit, 3 x 1 mg
For Use With (Application)Flow Cytometry, Fluoresence Microscopy
Product TypeLabeling Kit
Quantity3 x 1 mg Reactions
Detection MethodFluorescence
Excitation/Emission560/585 nm
Labeling Scale1 mg
Product LinepHrodo™
Chemical ReactivityAmine
Reactive MoietySTP Ester
SolubilityDMSO (Dimethylsulfoxide)
Label or DyepHrodo Red
ColorRed
Labeling TargetAntibodies, Proteins
Flow Cytometer Laser Lines561 nm
Label TypepHrodo
Unit Size3 x 1 mg reactions
P36022Full specifications
3 x 100 μg reactionsGreen Antibody Labeling Kit, 3 x 100 μg
Price: 595.00
Your price:
-
DescriptionGreen Antibody Labeling Kit, 3 x 100 μg
For Use With (Application)Flow Cytometry, Fluoresence Microscopy
Product TypeLabeling Kit
Quantity3 x 100 μg Reactions
Detection MethodFluorescence
Excitation/Emission505/525 nm
Labeling Scale100 μg
Product LinepHrodo™
Chemical ReactivityAmine
Reactive MoietySTP Ester
SolubilityDMSO (Dimethylsulfoxide)
Label or DyepHrodo Green
SpacerMid-length, Pegylated
ColorGreen
Labeling TargetAntibodies, Proteins
Flow Cytometer Laser Lines488 nm
Label TypepHrodo
Unit Size3 x 100 μg reactions
P36023Full specifications
3 x 1 mg reactionsGreen Antibody Labeling Kit, 3 x 1 mg
Price: 635.00
Your price:
-
DescriptionGreen Antibody Labeling Kit, 3 x 1 mg
For Use With (Application)Flow Cytometry, Fluoresence Microscopy
Product TypeLabeling Kit
Quantity3 x 1 mg Reactions
Detection MethodFluorescence
Excitation/Emission505/525 nm
Labeling Scale1 mg
Product LinepHrodo™
Chemical ReactivityAmine
Reactive MoietySTP Ester
SolubilityDMSO (Dimethylsulfoxide)
Label or DyepHrodo Green
SpacerMid-length, Pegylated
ColorGreen
Labeling TargetAntibodies, Proteins
Flow Cytometer Laser Lines488 nm
Label TypepHrodo
Unit Size3 x 1 mg reactions
P36015Full specifications
3 labelingsiFL Green Microscale Protein Labeling Kit, 3 labelings
Price: 710.00
Online price: 693.65
Your price:
-
DescriptioniFL Green Microscale Protein Labeling Kit, 3 labelings
For Use With (Application)Cell Analysis
Product TypeProtein Labeling Kit
Quantity1 Kit
Excitation/Emission505/525 nm
For Use With (Equipment)Flow Cytometer
Shipping ConditionRoom Temperature
Product LinepHrodo™
ColorGreen
Unit Size3 labelings
Showing 8 of 8

The pHrodo Red Antibody Labeling Kit and pHrodo Green Antibody Labeling Kit provide the reagents needed to easily label three purified protein or antibody samples with pHrodo Red or pHrodo Green dye. These kits contain amine-reactive, pH-sensitive pHrodo Red or Green STP ester dye that covalently binds to free lysines anywhere they occur in an antibody or protein.

The dyes included in this kit are improved versions of pHrodo Red and pHrodo Green dyes. They are optimized for the creation of bright and soluble bioconjugates that are used in the study of antibody internalization, endocytosis, phagocytosis, and for development of antibody-drug conjugates. These dyes are also more soluble than the original pHrodo Red and pHrodo Green dyes, making them useful for the labeling of antibodies that may otherwise precipitate out of solution during conjugation. pHrodo Red and pHrodo Green dyes are non-fluorescent outside the cell, but fluoresce bright red or bright green as the pH of their surroundings becomes more acidic, such as those found inside of phagosomes and endosomes.

The pHrodo Red and Green Microscale Labeling Kits offer the following benefits:
Convenience—Labeling typically takes one hour, with less than 30 minutes of hands-on time
Versatility—Labelling can be performed on 12-150 kDa molecular weight proteins
Customization—Create your own probes for studying antibody internalization, phagocytosis, and endocytosis

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

Contents & Storage

Store in refrigerator (2–8°C), desiccate, protect from light, and do not freeze.

Figures

Documents & Downloads

Certificates

    Frequently asked questions (FAQs)

    Citations & References

    Search citations by name, author, journal title or abstract text