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PrestoBlue™ HS Cell Viability Reagent, 100 mL - FAQs

PrestoBlue™ HS Cell Viability Reagent, 100 mL - FAQs

View additional product information for PrestoBlue™ and PrestoBlue™ HS Cell Viability Reagents - FAQs (P50200, P50201, A13262, A13261)

5 product FAQs found

Can I combine Invitrogen CellEvent Caspase-3/7 Green Detection Reagent with PrestoBlue Cell Viability Reagent?

Yes. Invitrogen CellEvent Caspase-3/7 Green Detection Reagent has been demonstrated in combination with Invitrogen PrestoBlue Cell Viability Reagent in the same sample. CellEvent Caspase reagent fluorescence reports activated caspase, while PrestoBlue Cell Viability Reagent reports cellular metabolic activity as an indicator of viability. Follow the instructions for each reagent and use single-reagent controls to evaluate signal interference.

What viability or cytotoxicity reagents would you recommend for a high throughput assay with multiple time points?

We recommend alamarBlue HS Cell Viability Reagent (Cat. No. A50101) or prestoBlue HS Cell Viability Reagent (Cat. Nos. P50200, P50201) for experiments with multiple time points or longer incubation time. We recommend that you add the reagent at each time-point at the end of each incubation period rather than continuous measurement. This prevents the reagent from being converted by metabolically active cells, which could confound results.

Use the link below to help you decide which reagent is most suitable for your purpose:

Microplate Assays for Cell Viability

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Why would I use PrestoBlue HS Cell Viability Reagent instead of the original PrestoBlue Cell Viability Reagent?

PrestoBlue HS Cell Viability Reagent provides a lower background, suitable for analyzing a lower number of cells per well. Also, the removal of impurities in the 'HS' reagent limits any artifacts that may be critical to your cells' viability.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Does PrestoBlue HS Cell Viability Reagent have the same properties as the original PrestoBlue Cell Viability Reagent?

Yes and in addition, PrestoBlue HS Cell Viability Reagent has a higher purity which results in a lower background.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the difference between PrestoBlue HS Cell Viability Reagent (Cat. Nos. P50200, P50201) and the original PrestoBlue Cell Viability Reagent (Cat. Nos. A13261, A13262)?

Both products utilize resazurin as the indicator dye. PrestoBlue HS Cell Viability Reagent undergoes a purification process that removes any residual resorufin that may be present from the standard manufacturing process for resazurin. This purification results in a product that has very low background. PrestoBlue HS Cell Viability Reagent may be used on samples with as few as 10 cells/well, compared to a minimum of 12 cells/well for the orginal PrestoBlue Cell Viability Reagent.
Note: Minimum cell number/well is also dependent upon reducing activity of the cell type. Results may vary.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.