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Invitrogen

nNOS Polyclonal Antibody

6 Published Figures
18 References
View all (34) nNOS antibodies

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nNOS Polyclonal Antibody

Product Details

PA1-033

Applications
Tested Dilution
Publications

Western Blot (WB)

1:100-1:1,000
View 11 publications 11 publications

Immunohistochemistry (IHC)

-
View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200
View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:50-1:500
View 3 publications 3 publications
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Pig, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues C N(1411) R L R S E S I A F I E E S K(1425) of human nNOS.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325021

Product Specific Information

PA1-033 detects nNOS from human, rat and mouse samples.

PA1-033 has been successfully used in Western blot, ICC/IF and immunohistochemisty (paraffin) procedures. By Western blot, this antibody detects an ~160 kDa protein. Detects a few non-specific bands in Western blot analysis of rat and mouse brain lysates. This antibody is not recommended for MCF-7, PC-12, SK-N-C or U87-MG cells in Western blot applications.

The PA1-033 immunogen is a synthetic peptide to residues C N(1411) R L R S E S I A F I E E S K(1425) of human nNOS. The peptide sequence is 100% conserved in rat, mouse, frog, and rabbit nNos proteins. This peptide (Cat. # PEP-190) is available for use in neutralization and control experiments.

Target Information

NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: bNOS1; Brain NOS; constitutive NOS; N-NOS; NC-NOS; neuronal nitric oxide synthase; neuronal NOS; nitric oxidase synthase; nitric oxide synthase 1 (neuronal); nitric oxide synthase 1, neuronal; nitric oxide synthase, brain; nNOS; nNOS mu; NOS Type 1; NOS type I; peptidyl-cysteine S-nitrosylase NOS1; Phospho-bNOS; Phospho-Brain NOS; unnamed protein product

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Gene Aliases: 2310005C01Rik; bNOS; IHPS1; N-NOS; NC-NOS; nNOS; NO; NOS; Nos-1; NOS-I

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UniProt ID: (Human) P29475, (Mouse) Q9Z0J4, (Rat) P29476

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Entrez Gene ID: (Human) 4842, (Mouse) 18125, (Rat) 24598

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Function(s)
nitric-oxide synthase activity calcium channel regulator activity protein binding calmodulin binding FMN binding oxidoreductase activity sodium channel regulator activity heme binding tetrahydrobiopterin binding arginine binding peptidyl-cysteine S-nitrosylase activity ion channel binding cadmium ion binding calcium-dependent protein binding flavin adenine dinucleotide binding NADP binding scaffold protein binding zinc ion binding enzyme binding identical protein binding ATPase binding phosphoprotein binding RNA polymerase II sequence-specific DNA binding transcription factor binding NADPH binding
Process(es)
response to hypoxia regulation of the force of heart contraction regulation of sodium ion transport system process arginine catabolic process nitric oxide biosynthetic process striated muscle contraction cell communication negative regulation of cell proliferation response to heat response to hormone peptidyl-cysteine S-nitrosylation signaling response to lipopolysaccharide multicellular organismal response to stress negative regulation of iron ion transmembrane transport nitric oxide-cGMP-mediated signaling pathway xenobiotic catabolic process vasodilation negative regulation of apoptotic process negative regulation of potassium ion transport response to peptide hormone negative regulation of neuron apoptotic process positive regulation of neuron apoptotic process response to estrogen establishment of protein localization positive regulation of G-protein coupled receptor protein signaling pathway negative regulation of blood pressure positive regulation of transcription, DNA-templated negative regulation of vasoconstriction positive regulation of transcription from RNA polymerase II promoter negative regulation of insulin secretion behavioral response to cocaine regulation of neurogenesis negative regulation of hydrolase activity negative regulation of cytosolic calcium ion concentration negative regulation of serotonin uptake negative regulation of calcium ion transport regulation of postsynaptic membrane potential negative regulation of hepatic stellate cell contraction cellular response to mechanical stimulus cellular response to growth factor stimulus cellular response to epinephrine stimulus positive regulation of adrenergic receptor signaling pathway positive regulation of the force of heart contraction synaptic signaling by nitric oxide positive regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway positive regulation of long-term synaptic potentiation positive regulation of sodium ion transmembrane transport myoblast fusion blood circulation negative regulation of calcium ion transport into cytosol regulation of cardiac muscle contraction by calcium ion signaling regulation of metal ion transport negative regulation of ion transport cell redox homeostasis regulation of cardiac muscle contraction regulation of calcium ion transmembrane transport via high voltage-gated calcium channel regulation of blood circulation positive regulation of membrane repolarization during ventricular cardiac muscle cell action potential female pregnancy response to mechanical stimulus response to lead ion response to activity response to nutrient levels response to vitamin E response to vitamin B3 response to nicotine response to decreased oxygen levels regulation of apoptotic process response to ethanol nitric oxide metabolic process response to nitric oxide
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