Search

  • Contáctenos
  • Orden Rápida

Invitrogen

Leptin Polyclonal Antibody

Advanced Verification
13 Published Figures
14 References
View all (33) Leptin antibodies

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Additional Information:
{{banner.description}}
{{$ctrl.isSkuNotesCollapsed ? 'View more' : 'View less'}}
Leptin Polyclonal Antibody

Product Details

PA1-051

Applications
Tested Dilution
Publications

Western Blot (WB)

1:200-1:1,000
View 10 publications 10 publications

Immunohistochemistry (IHC)

-
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

-
View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:50 - 1:200
-

Immunocytochemistry (ICC/IF)

2 µg/mL
-

ELISA (ELISA)

-
View 1 publication 1 publication

ChIP assay (ChIP)

1.5 µg/1x10^6 cells
-

Immunomicroscopy (IM)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human, Mouse, Sheep, Rat

Published species

Avian, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues Q(25) K V Q D D T K T L I K T I V T R I N D(44) of mouse Leptin.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2 mg/mL

Amount

100 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325786

Product Specific Information

PA1-051 detects leptin from human, mouse, sheep and rat samples.

PA1-051 has been successfully used in Western blot, immunocytochemistry and immunohistochemistry experiments. By Western blot, this antibody detects an ~16 kDa protein representing leptin from 3T3-L1 cell extract. Immunohistochemical staining of leptin in sheep brain with PA1-051 results in staining of the ventromedial hypothalamus.

The PA1-051 immunogen is a synthetic peptide corresponding to residues Q(25) K V Q D D T K T L I K T I V T R I N D(44) of mouse Leptin. This sequence is completely conserved between mouse and bovine.

Target Information

Leptin is a 167 amino acid long protein hormone with important effects in regulating body weight, metabolism and reproductive function. Leptin is approximately 16 kDa in mass and encoded by the obese (ob) gene. Leptin is expressed predominantly by adipocytes, which fits with the idea that body weight is sensed as the total mass of fat in the body. Smaller amounts of leptin are also secreted by cells in the epithelium of the stomach and in the placenta. Leptin receptors are highly expressed in areas of the hypothalamus known to be important in regulating body weight, as well as in T lymphocytes and vascular endothelial cells. Leptin's effects on body weight are mediated through effects on hypothalamic centers that control feeding behavior and hunger, body temperature and energy expenditure. Leptin is involved in regulating food intake, energy expenditure, and adiposity through hypothalamic leptin receptors. Leptin promotes hematopoiesis, angiogenesis, wound healing, inflammation, immune responses, influences pubertal development and fetal growth. Studies have investigated the role of leptin in obesity, anorexia nervosa, insulin resistance, and hypertension. Leptin also has thermogenic actions and regulates enzymes of fatty acid oxidation. Severe hereditary obesity in rodents and humans can be caused by defects in leptin production.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CD295 antigen; H-Leptin; HuB219; LEP-R; Leptin; leptin (murine obesity homolog); leptin (obesity homolog, mouse); ob; OB-R; obese; Obese protein; obese, mouse, homolog of; obesity; Obesity factor; obesity protein; unnamed protein product

View more View less

Gene Aliases: LEP; LEPD; OB; obese; OBS

View more View less

UniProt ID: (Human) P41159, (Rat) P50596, (Mouse) P41160

View more View less

Entrez Gene ID: (Human) 3952, (Sheep) 443534, (Rat) 25608, (Mouse) 16846

View more View less

Function(s)
DNA binding receptor binding hormone activity protein binding peptide hormone receptor binding leptin receptor binding cytokine activity growth factor activity peptide hormone
Process(es)
negative regulation of transcription from RNA polymerase II promoter angiogenesis ovulation from ovarian follicle response to hypoxia positive regulation of cytokine production placenta development regulation of endothelial cell proliferation response to dietary excess cardiac muscle hypertrophy glucose metabolic process regulation of gluconeogenesis energy reserve metabolic process glycerol biosynthetic process lipid metabolic process fatty acid beta-oxidation phagocytosis signal transduction JAK-STAT cascade female pregnancy response to nutrient circadian rhythm cholesterol metabolic process bile acid metabolic process regulation of blood pressure positive regulation of cell proliferation determination of adult lifespan adult feeding behavior fatty acid catabolic process negative regulation of metabolic process negative regulation of autophagy negative regulation of lipid storage response to activity regulation of metabolic process sexual reproduction central nervous system neuron development insulin secretion T cell differentiation regulation of intestinal cholesterol absorption response to nutrient levels positive regulation of TOR signaling negative regulation of appetite prostaglandin secretion response to estradiol positive regulation of interleukin-12 production positive regulation of interleukin-6 production positive regulation of interleukin-8 production positive regulation of tumor necrosis factor production regulation of natural killer cell activation regulation of natural killer cell proliferation response to insulin cellular response to insulin stimulus response to vitamin E leptin-mediated signaling pathway positive regulation of luteinizing hormone secretion positive regulation of peroxisome proliferator activated receptor signaling pathway intracellular signal transduction bone mineralization involved in bone maturation aorta development negative regulation of appetite by leptin-mediated signaling pathway positive regulation of T cell proliferation regulation of natural killer cell mediated cytotoxicity positive regulation of protein import into nucleus hormone metabolic process glucose homeostasis eating behavior negative regulation of apoptotic process positive regulation of ion transport positive regulation of MAPK cascade cellular response to leptin stimulus response to ethanol regulation of fat cell differentiation regulation of angiogenesis negative regulation of vasoconstriction negative regulation of glucose import positive regulation of JAK-STAT cascade positive regulation of insulin receptor signaling pathway regulation of bone remodeling positive regulation of follicle-stimulating hormone secretion regulation of lipid biosynthetic process positive regulation of developmental growth regulation of insulin secretion regulation of steroid biosynthetic process intestinal absorption leukocyte tethering or rolling regulation of nitric-oxide synthase activity negative regulation of transport elastin metabolic process regulation of cell cycle positive regulation of protein kinase B signaling regulation of lipoprotein lipid oxidation adipose tissue development negative regulation of cartilage development negative regulation of glucagon secretion cellular response to L-ascorbic acid cellular response to retinoic acid regulation of brown fat cell differentiation energy homeostasis receptor signaling pathway via STAT bone growth positive regulation of cold-induced thermogenesis regulation of cytokine production involved in inflammatory response regulation of protein localization to nucleus positive regulation of p38MAPK cascade positive regulation of fat cell apoptotic process activation of protein kinase C activity positive regulation of reactive oxygen species metabolic process negative regulation of glutamine transport positive regulation of hepatic stellate cell activation regulation of protein phosphorylation tyrosine phosphorylation of STAT protein cAMP-mediated signaling positive regulation of tyrosine phosphorylation of Stat3 protein positive regulation of myeloid cell differentiation positive regulation of STAT protein import into nucleus
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support