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Invitrogen
alpha Internexin Polyclonal Antibody
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FIGURE: 1 / 2
alpha Internexin Antibody (PA1-10009) in ICC/IF
Immunofluorescent analysis of alpha Internexin in a rat cerebellum section. The rat cerebellum section was obtained following transcardial perfusion of the rat with 4% paraformaldehyde, brain was post fixed for 24 hours, and cut to 45µM. Free-floating sections were stained with an alpha internexin polyclonal antibody (Product # PA1-10009) at a dilution of 1:5,000 as seen in red, and costained with an MBP po... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA1-10009
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Horse,
Human,
Mouse,
Pig,
Rat
Host/Isotype
Chicken
/ IgY
Class
Polyclonal
Type
Antibody
Immunogen
Full length recombinant rat ?-internexin expressed in and purified from E. coli.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
In Western blot, human proteins run at ~68 kDa, while rat and mouse run lower at ~66 kDa. Minor bands at ~150 kDa are probably covalent dimers, and bands at ~50 kDa represent alpha-internexin breakdown products.
Stable at 4°C for at least one year. Mix 1:1 with 100% glycerol and store at -20°C for longer term storage.
Target Information
alpha-Internexin is a Class IV intermediate filament subunit expressed very early in neuronal development. It is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. Many classes of mature neurons contain a-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons a-internexin is the only neurofilament subunit expressed. Antibodies to internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition the very early developmental expression of a-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage. The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 66 kDa neurofilament protein; alpha-Inx; FLJ18662; FLJ57501; MGC12702; Neuro; neurofilament 5 (66kD); Neurofilament 66; neurofilament-66; neurofilament-66, tax-binding protein; unnamed protein product
Gene Aliases: alpha-Inx; Inexa; Intlaa; NEF5; NF-66; NF66; TXBP-1
UniProt ID: (Human) Q16352, (Bovine) Q08DH7, (Mouse) P46660
Entrez Gene ID: (Pig) 100153400, (Horse) 100059769, (Human) 9118, (Bovine) 532236, (Mouse) 226180, (Rat) 24503
intermediate filament cytoskeleton organization
intermediate filament organization
neurofilament cytoskeleton organization
postsynaptic modulation of chemical synaptic transmission
postsynaptic intermediate filament cytoskeleton organization
cellular response to leukemia inhibitory factor
tissue regeneration
substantia nigra development
nervous system development
cell differentiation
postsynaptic actin cytoskeleton organization
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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