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Invitrogen
NEFL Polyclonal Antibody
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FIGURE: 1 / 5
NEFL Antibody (PA1-10014) in ICC/IF
Immunofluorescent analysis of NEFL in mouse cerebellum section. The mouse brain section was obtained following transcardial perfusion of the mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, and cut to 45µM. Free-floating sections were stained using an NEFL polyclonal antibody (Product # PA1-10014) at a dilution of 1:5,000 as seen in red, and costained using a MBP polyclonal antibody (Product # PA1-10008) at a dilution of 1:5,000 as seen in green. The NEFL antibody labels dendrites and axons of neuronal cells, and the MBP... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA1-10014
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse,
Pig,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant full length human NF-L protein
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
5mM sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
For Western blot using alkaline phosphatase plus chromagen, a dilution of at least 1:1000 is recommended. For chemiluminescence, use at a dilution of 1:20,000.
Stable at 4°C for at least one year. Mix 1:1 with 100% glycerol and store at -20°C for longer term storage.
Target Information
Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-L is the light or low molecular weight microfilament subunit and runs on SDS-PAGE gels at approximately 70 kDa. Neurofilament are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the heavy or high molecular weight microfilament subunit and runs on SDS-PAGE gels in the range 180-220 kDa, with some variation in different species.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 68 kDa neurofilament protein; 68kDa neurofilament; FLJ53642; intermediate filament; light molecular weight neurofilament protein; micro glutamic acid-rich protein; N-FL; NEF3; NEFL; neurofilament; neurofilament 3; neurofilament protein; neurofilament protein, light chain; neurofilament subunit NF-L; Neurofilament triplet L protein; neurofilament, light polypeptide; neurofilament, light polypeptide 68kDa; neurofilament-l; neurofilament-light; NF-L; NF-M; NFM; NLC; OTTHUMP00000196614; protein phosphatase 1, regulatory subunit 110; unnamed protein product
Gene Aliases: CMT1F; CMT2E; CMTDIG; NF-L; NF68; NFL; PPP1R110
UniProt ID: (Bovine) P02548, (Human) P07196, (Rat) P19527, (Mouse) P08551
Entrez Gene ID: (Bovine) 281348, (Human) 4747, (Pig) 100521224, (Rat) 83613, (Mouse) 18039
structural constituent of cytoskeleton
protein C-terminus binding
protein binding, bridging
identical protein binding
structural constituent of postsynaptic intermediate filament cytoskeleton
protein binding
tubulin binding
protein domain specific binding
phospholipase binding
macromolecular complex binding
molecular condensate scaffold activity
microtubule cytoskeleton organization
anterograde axonal transport
retrograde axonal transport
peripheral nervous system axon regeneration
axonal transport of mitochondrion
regulation of axon diameter
neurofilament bundle assembly
locomotion
negative regulation of neuron apoptotic process
intermediate filament organization
intermediate filament bundle assembly
neuron projection morphogenesis
positive regulation of axonogenesis
neuromuscular process controlling balance
neurofilament cytoskeleton organization
synapse maturation
postsynaptic intermediate filament cytoskeleton organization
response to toxic substance
spinal cord development
hippocampus development
cerebral cortex development
response to peptide hormone
intermediate filament polymerization or depolymerization
protein polymerization
response to corticosterone
regulation of synapse maturation
postsynaptic modulation of chemical synaptic transmission
response to sodium arsenite
response to acrylamide
axon development
intermediate filament polymerization
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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