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NF-H Polyclonal Antibody
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FIGURE: 1 / 6
NF-H Antibody (PA1-10016) in WB
Western blot analysis of NF-H in tissue lysates using an NF-H polyclonal antibody (Product # PA1-10016) at a dilution of 1:10,000 as seen in green. 1) protein standard (red), 2) rat brain, 3) rat spinal cord 4) mouse brain, and 5) mouse spinal cord lysate. A strong band at about 220 kDa corresponds to the phosphorylated axonal form of the NF-H subunit. Smaller proteolytic fragments of NF-H are also detected with the antibody.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA1-10016
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Horse,
Human,
Mouse,
Pig,
Rat
Published species
Human,
Whale
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Native NF-H purified from bovine spinal cord
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
5mM sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-10016 detects Neurofilament NF-H, primarily the hyperphosphorylated axonal form which yields a band at ~200kDa in Western blot applications.
Target Information
Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-H has an important role in mature axons and is involved in amyotrophic lateral sclerosis (ALS).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 200 kDa neurofilament protein; heavy neurofilament polypeptide; heavy neurofilament subunit; high molecular weight neurofilament; hSTEp; NEF3; neurofilament; neurofilament 200kDa; neurofilament heavy polypeptide; Neurofilament triplet H protein; neurofilament, heavy polypeptide; neurofilament, heavy polypeptide 200kDa; NF 200 kD; NF-H; Nfh; NF200; NHC; unnamed protein product
Gene Aliases: CMT2CC; mKIAA0845; NF-H; NF200; NFH
UniProt ID: (Human) P12036, (Mouse) P19246
Entrez Gene ID: (Horse) 100064328, (Human) 4744, (Pig) 100156492, (Mouse) 380684, (Rat) 24587
microtubule cytoskeleton organization
ovarian follicle atresia
axonogenesis
axon regeneration
neurofilament bundle assembly
intermediate filament cytoskeleton organization
intermediate filament bundle assembly
peripheral nervous system neuron axonogenesis
neurofilament cytoskeleton organization
axon development
postsynaptic modulation of chemical synaptic transmission
postsynaptic intermediate filament cytoskeleton organization
regulation of organelle transport along microtubule
response to sodium arsenite
cellular response to leukemia inhibitory factor
cell projection assembly
spinal cord development
hippocampus development
cerebral cortex development
neuron projection development
cellular response to oxidative stress
response to cocaine
cellular response to estradiol stimulus
cellular response to hydroperoxide
response to acrylamide
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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