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Invitrogen
Ubiquitin Polyclonal Antibody
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FIGURE: 1 / 4
Ubiquitin Antibody (PA1-10023) in ICC/IF
Immunofluorescent analysis of Ubiquitin in HeLa cells. Cells were stained with a ubiquitin polyclonal antibody (Product # PA1-10023) at a dilution of 1:1,000 as seen in red, stained with a Vimentin polyclonal antibody (Product # PA1-10003) at a dilution of 1:10,000 as seen in green, and with DAPI staining the nuclear DNA as seen in blue. A) Control HeLa cells maintained in normal medium, B) HeLa cells treat... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA1-10023
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
All,
Human,
Mouse,
Rat
Published species
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Purified ubiquitin conjugated with glutaraldehyde to KLH
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
5mM sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-10023 was made against purified bovine blood derived ubiquitin coupled to keyhole limpet hemocyanin with glutaraldehyde.
Target Information
Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi. Multiple alternatively spliced transcript variants that encode the same proteins have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 40S ribosomal protein S27-like; 40S ribosomal protein S27-like protein; 40S ribosomal protein S27a; 60S ribosomal protein L40; CEP52; Chap1; epididymis luminal protein 112; epididymis secretory protein Li 50; hPLIC-2; HRIHFB2157; HUBCEP52; OTTHUMP00000200786; OTTHUMP00000201692; PLIC-2; polyubiquitin B; polyubiquitin C; pro-ubiquitin; ribosomal protein S27; ribosomal protein S27-like 1; RPL40; RPS27A; small ribosomal subunit protein eS27-like; UBA52; UBB; UBC; Ubiquilin 2; ubiquitin; ubiquitin and ribosomal protein S27a; ubiquitin carboxyl extension protein 52; ubiquitin carboxyl extension protein 80; ubiquitin precursor; ubiquitin-40S ribosomal protein S27a; ubiquitin-52 amino acid fusion protein; ubiquitin-60S ribosomal protein L40; ubiquitin-CEP52; ubiquitin-CEP80; ubiquitin/60S ribosomal fusion protein; unnamed protein product
Gene Aliases: 0610006J14Rik; 1810034D23Rik; 2700054O04Rik; CEP52; CEP80; D8Ertd21e; eS31; Gm1863; HEL-S-50; HEL112; HMG20; HUBCEP52; L40; RPL40; Rps27a; Rps27l1; S27A; TI-225; Uba52; UBA80; Ubb; Ubb2; Ubc; UBCEP1; UBCEP80
translation
biological_process
protein ubiquitination
modification-dependent protein catabolic process
ribosomal small subunit biogenesis
cytoplasmic translation
striated muscle contraction
spermatogenesis
translation at presynapse
translation at postsynapse
negative regulation of myoblast fusion
rRNA processing
DNA damage response, signal transduction by p53 class mediator
mitotic G1 DNA damage checkpoint
intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
positive regulation of translation
response to insecticide
protein modification process
male meiosis I
female meiosis I
male gonad development
female gonad development
hypothalamus gonadotrophin-releasing hormone neuron development
positive regulation of protein ubiquitination
regulation of neuron apoptotic process
mitochondrion transport along microtubule
neuron projection morphogenesis
regulation of mitochondrial membrane potential
adipose tissue development
fat pad development
regulation of proteasomal protein catabolic process
seminiferous tubule development
energy homeostasis
positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator
positive regulation of protein monoubiquitination
ubiquitin-dependent protein catabolic process
protein homotetramerization
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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