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Invitrogen
PGP9.5 Polyclonal Antibody
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FIGURE: 1 / 8
PGP9.5 Antibody (PA1-10024) in ICC/IF
Immunofluorescent analysis of PGP9.5 in SH-SY5Y cells. The cells were stained using a UCHL1 polyclonal antibody (Product # PA1-10024) at a dilution of 1:1,000 as seen in green, with a fibrillarin monoclonal antibody at a dilution of 1:1,000 as seen in red, and with DAPI staining the nuclear DNA in blue. The UCHL1 antibody produces strong staining of the cellular cytoplasm of these cells which share many properties with neurons, while the fibrillarin antibody specifically labels nucleoli.
Product Details
PA1-10024
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Chicken,
Horse,
Human,
Mouse,
Pig,
Rat
Published species
Deer
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Human UCHL1 expressed in and purified from E. coli.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
5mM sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-10024 was made against full length recombinant human UCHL1 expressed in and purified from E. coli and can be used to identify neurons and their processes in culture or in sections. The antibody works cleanly on appropriate lysates of cell and tissues.
Target Information
PGP9.5 (Protein gene product 9.5, UCH-L1, PARK5) is a neuron specific protein, structurally and immunologically distinct from neuron specific enolase. PGP9.5 has a molecular weight of 27 kDa and was first identified by high resolution two dimensional PAGE. PGP9.5 is a member of ubiquitin carboxyl-terminal hydrolase family 1 (peptidase family C12) with a ubiquitin carboxyl-terminal hydrolase domain. PGP9.5 is well known for having ubiquitin hydrolase and ligase activities that hydrolyzes small C-terminal adducts of ubiquitin to generate ubiquitin monomers. PGP9.5 is present in neurons and nerve fibers at all levels of the central and peripheral nervous system, in neuroendocrine cells, in segments of the renal tubules, in spermatogonia and Leydig cells of the testis, in ova and in some cells of both the pregnant and non-pregnant corpus luteum. Over expression of PGP9.5 leads to non-small cell lung cancer while decreased expression leads to Huntington disease and Alzheimer disease. Since PGP9.5 is present in cellular inclusions, it can be a useful as a neuronal marker and in the studies of neurodegenerative disorders such as with Parkinson disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: epididymis luminal protein 117; epididymis secretory protein Li 53; gracile axonal dystrophy; Neuron cytoplasmic protein 9.5; OTTHUMP00000218137; OTTHUMP00000218139; OTTHUMP00000218140; OTTHUMP00000218141; PGP 9.5; PGP9.5; protein gene product 9.5; ubiquitin C-terminal hydrolase 1; ubiquitin carboxy-terminal hydrolase L1; ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase); Ubiquitin carboxyl-terminal hydrolase isozyme L1; ubiquitin carboxyl-terminal hydrolase L1; Ubiquitin thioesterase L1; ubiquitin thiolesterase; ubiquitin thiolesterase L1; UCH-L1; UCHL; unnamed protein product
Gene Aliases: AW822034; C88048; gad; HEL-117; HEL-S-53; NDGOA; PARK5; PGP 9.5; PGP9.5; PGP95; R75593; SPG79; SPG79A; UCH-L1; UCHL-1; UCHL1
UniProt ID: (Pig) Q6SEG5, (Human) P09936, (Horse) Q9GM50, (Bovine) P23356, (Mouse) Q9R0P9, (Rat) Q00981
Entrez Gene ID: (Pig) 396637, (Human) 7345, (Chicken) 770302, (Horse) 100033838, (Bovine) 514394, (Mouse) 22223, (Rat) 29545
cysteine-type endopeptidase activity
ligase activity
ubiquitin protein ligase binding
alpha-2A adrenergic receptor binding
ubiquitin binding
thiol-dependent ubiquitin-specific protease activity
protein binding
peptidase activity
cysteine-type peptidase activity
omega peptidase activity
hydrolase activity
receptor inhibitor activity
ribosome binding
cysteine protease
response to ischemia
ubiquitin-dependent protein catabolic process
axon target recognition
adult walking behavior
cell proliferation
axonal transport of mitochondrion
cellular response to drug
eating behavior
negative regulation of MAP kinase activity
muscle fiber development
neuromuscular process
male germ cell proliferation
proteolysis
axonogenesis
regulation of macroautophagy
protein deubiquitination
protein catabolic process
proteasome-mediated ubiquitin-dependent protein catabolic process
negative regulation of MAPK cascade
positive regulation of glycolytic process
muscle cell development
cellular response to xenobiotic stimulus
sensory perception of pain
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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