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Invitrogen
Phospho-IRS1 (Ser307) Polyclonal Antibody
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FIGURE: 1 / 5
Phospho-IRS1 (Ser307) Antibody (PA1-1054) in ICC/IF
Immunofluorescence analysis of Phospho-IRS1 pSer307 was done on 70% confluent log phase T-47D cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Phospho-IRS1 pSer307 Rabbit Polyclonal Antibody (Product # PA1-1054) at 1 µg/mL in 1% BSA and incubated for ... View More
Product Details
PA1-1054
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Horse,
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic phosphopeptide corresponding to residues E(301) S I T A T (pS) P A S M V G G K(315) of human IRS-1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-1054 detects phosphorylated IRS-1 (Ser307) in mouse and human cells.
PA1-1054 has been successfully used in Western Blot procedures. By WB, this antibody detects an ~170 kDa protein representing phospho-IRS-1 (Ser307) from 3T3 L1 cells treated with insulin for 10 minutes.
The PA1-1054 immunogen is a synthetic phosphopeptide corresponding to residues E(301) S I T A T (pS) P A S M V G G K(315) of human IRS-1. This peptide is 100% conserved in rats and mice. This peptide (Cat. #PEP-184) is available for use in neutralization and control experiments.
Target Information
IRS-1, a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Insulin receptor substrate 1; IRS-1
Gene Aliases: G972R; HIRS-1; IRS-1; IRS1
UniProt ID: (Human) P35568, (Mouse) P35569
Entrez Gene ID: (Human) 3667, (Mouse) 16367
phosphotyrosine binding
transmembrane receptor protein tyrosine kinase adaptor activity
protein kinase C binding
receptor binding
insulin receptor binding
insulin-like growth factor receptor binding
protein binding
receptor signaling complex scaffold activity
signaling adaptor activity
SH2 domain binding
phosphatidylinositol 3-kinase binding
signal transducer activity
protein kinase binding
protein domain specific binding
signal transduction
positive regulation of cell proliferation
insulin receptor signaling pathway
positive regulation of signal transduction
positive regulation of glucose metabolic process
cytokine-mediated signaling pathway
positive regulation of fatty acid beta-oxidation
response to insulin
cellular response to insulin stimulus
glucose homeostasis
protein kinase B signaling
positive regulation of glycogen biosynthetic process
positive regulation of glucose import
negative regulation of insulin receptor signaling pathway
positive regulation of insulin receptor signaling pathway
negative regulation of insulin secretion
insulin-like growth factor receptor signaling pathway
positive regulation of protein kinase B signaling
cellular response to fatty acid
positive regulation of mesenchymal cell proliferation
regulation of gene expression
phosphatidylinositol 3-kinase signaling
lipid catabolic process
insulin secretion
positive regulation of cell migration
mammary gland development
response to caffeine
protein localization to nucleus
positive regulation of phosphorylation
response to peptide hormone
positive regulation of phosphatidylinositol 3-kinase activity
protein heterooligomerization
positive regulation of glucagon secretion
negative regulation of somatostatin secretion
positive regulation of glucose import in response to insulin stimulus
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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