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AgRP Polyclonal Antibody
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AgRP Antibody (PA1-18414) in IHC (P)
Immunohistochemistry was performed on 4% paraformaldehyde-fixed paraffin-embedded mouse brain tissue sections. To expose target antigens, tissues were treated with 15 µg/mL Proteinase K in PBS containing 0.01% Triton X-100 for 5 minutes. Tissues were blocked for 1 hour and probed with an Agouti Related Protein polyclonal antibody (Product # PA1-18414) at a dilution of 1:200 overnight at 4°C. Tissues were wa... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA1-18414
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Sheep,
Rat
Published species
Mouse
Host/Isotype
Guinea pig
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to a region (82-131) within the carboxy domain of mouse agouti related protein conjugated to carrier protein.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute in 50 µL sterile water. Centrifuge to remove any insoluble material. Store lyophilized antibody at 2-8 °C. It is recommended that a thawed sample is stored at 4 °C for no longer than 2 weeks. Allocation of appropriate anti-bacterial agent can increase shelf life by several weeks. Diluted serum should be prepared as required. Long term stability requires storage, preferably in small aliquots at -20 °C or lower. Glycerol (1:1) can be added to neat serum for additional stability if intended use does not prevent this.
Target Information
Agouti-related protein (AgRP), also called agouti-related peptide, is a neuropeptide produced in the brain by the AgRP/NPY neuron. In humans, the agouti-related peptide is encoded by the AGRP gene. This gene encodes an antagonist of the melanocortin-3 and melanocortin-4 receptor. It appears to regulate hypothalamic control of feeding behavior via melanocortin receptor and/or intracellular calcium regulation, and thus plays a role in weight homeostasis. Mutations in this gene have been associated with late on-set obesity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: agouti related protein homolog; AGRP; orexigenic neuropeptide
Gene Aliases: AGRT; ART; ASIP2
UniProt ID: (Human) O00253, (Mouse) P56473
Entrez Gene ID: (Sheep) 443269, (Human) 181, (Mouse) 11604, (Rat) 25582
neuropeptide signaling pathway
circadian rhythm
adult feeding behavior
hormone-mediated signaling pathway
regulation of appetite
positive regulation of appetite
response to insulin
eating behavior
long-day photoperiodism
maternal process involved in female pregnancy
regulation of feeding behavior
negative regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway
positive regulation of feeding behavior
positive regulation of eating behavior
feeding behavior
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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