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          • Primary Antibodies ›
          • IL-6 Antibodies

          Invitrogen

          IL-6 Polyclonal Antibody

          2 Published Figures
          3 References
          View all (203) IL-6 antibodies

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          Cite IL-6 Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (2)
          • Published Figures (2)
          IL-6 Antibody in Western Blot (WB)
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          IL-6 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 4

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          IL-6 Antibody (PA1-26811) in WB

          Western blot was performed using Anti-IL-6 Polyclonal Antibody (Product # PA1-26811) and a 24 kDa band corresponding to IL-6 was observed across to be upregulated upon treatment of HUVEC cells with LPS and Brefeldin A. Whole cell extracts (50 µg lysate) of HUVEC (Lane 1) and HUVEC treated with LPS and Brefeldin A (0.5 µg/mL LPS for 24 h and 300 ng/mL Brefeldin A for last 20 h of LPS stimulation) (Lane 2) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitro... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          IL-6 Antibody in Western Blot (WB)
          IL-6 Antibody in Western Blot (WB)
          IL-6 Antibody in Immunohistochemistry (IHC)
          IL-6 Antibody in Immunohistochemistry (IHC)

          Product Details

          PA1-26811

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000
          -

          Immunohistochemistry (IHC)

          -
          View 2 publications 2 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:1:100-1:200
          View 1 publication 1 publication

          Immunohistochemistry (Frozen) (IHC (F))

          Assay-Dependent
          -

          ELISA (ELISA)

          1:500-1:2,000
          -

          Neutralization (Neu)

          1:100
          -

          Dot blot (DB)

          Assay-dependent
          -
          Product Specifications

          Species Reactivity

          Human

          Published species

          Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant human IL-6 produced in E.coli.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          80 mg/mL

          Storage buffer

          PBS, pH 7.2

          Contains

          no preservative

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_794649

          Product Specific Information

          This antibody detects recombinant and native IL-6 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-6 production from fibroblasts). In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule.

          Target Information

          IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: B-cell differentiation factor; B-cell stimulatory factor 2; BSF-2; CDF; CTL differentiation factor; H-IL-6; Hybridoma growth factor; hybridoma growth factor peptide; IFN-beta-2; IL-6; IL6; ILN; interferon 6 precursor; Interferon beta-2; Interleukin; interleukin 6; interleukin BSF-2; Interleukin-6; Interleukin6; M-IL-6; R-IL-6; unnamed protein product

          View more View less

          Gene Aliases: BSF-2; BSF2; CDF; HGF; HSF; IFN-beta-2; IFNB2; IL-6; IL6

          View more View less

          UniProt ID: (Human) P05231

          View more View less

          Entrez Gene ID: (Human) 3569

          View more View less

          Function(s)
          cytokine activity interleukin-6 receptor binding protein binding growth factor activity identical protein binding
          Process(es)
          neutrophil apoptotic process germinal center B cell differentiation hepatic immune response neutrophil mediated immunity monocyte chemotaxis positive regulation of immunoglobulin production positive regulation of acute inflammatory response positive regulation of leukocyte chemotaxis acute-phase response inflammatory response immune response humoral immune response JAK-STAT cascade positive regulation of cell proliferation negative regulation of cell proliferation vascular endothelial growth factor production regulation of vascular endothelial growth factor production positive regulation of vascular endothelial growth factor production positive regulation of gene expression positive regulation of epithelial to mesenchymal transition negative regulation of lipid storage response to activity cytokine-mediated signaling pathway cell differentiation platelet activation endocrine pancreas development neuron projection development response to peptidoglycan negative regulation of chemokine production positive regulation of chemokine production positive regulation of interleukin-1 beta production positive regulation of interleukin-10 production positive regulation of interleukin-17 production positive regulation of interleukin-21 production positive regulation of interleukin-6 production positive regulation of interleukin-8 production positive regulation of tumor necrosis factor production negative regulation of collagen biosynthetic process positive regulation of peptidyl-serine phosphorylation maintenance of blood-brain barrier positive regulation of T cell proliferation glucose homeostasis positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of MAPK cascade negative regulation of fat cell differentiation positive regulation of osteoblast differentiation positive regulation of translation regulation of angiogenesis negative regulation of bone resorption positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of JAK-STAT cascade positive regulation of smooth muscle cell proliferation positive regulation of peptidyl-tyrosine phosphorylation negative regulation of neurogenesis regulation of insulin secretion defense response to Gram-negative bacterium defense response to Gram-positive bacterium positive regulation of B cell activation regulation of protein transport positive regulation of lymphocyte activation response to glucocorticoid defense response to virus positive regulation of glial cell proliferation T follicular helper cell differentiation regulation of astrocyte activation neuron cellular homeostasis glucagon secretion regulation of glucagon secretion interleukin-6-mediated signaling pathway cellular response to hydrogen peroxide cellular response to lipopolysaccharide T-helper 17 cell lineage commitment hepatocyte proliferation positive regulation of extracellular matrix disassembly inflammatory response to wounding liver regeneration receptor signaling pathway via STAT cellular response to virus regulation of neuroinflammatory response positive regulation of neuroinflammatory response positive regulation of cytokine production involved in inflammatory response positive regulation of platelet aggregation positive regulation of apoptotic DNA fragmentation positive regulation of pri-miRNA transcription from RNA polymerase II promoter regulation of microglial cell activation positive regulation of STAT cascade positive regulation of leukocyte adhesion to vascular endothelial cell positive regulation of T-helper 2 cell cytokine production negative regulation of primary miRNA processing negative regulation of interleukin-1-mediated signaling pathway positive regulation of type B pancreatic cell apoptotic process
          It has to be done as per old AB suggested Products section.

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