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Invitrogen
Phospho-ERK1/ERK2 (Thr202, Tyr204) Polyclonal Antibody
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FIGURE: 1 / 5
Phospho-ERK1/ERK2 (Thr202, Tyr204) Antibody (PA1-4607) in ICC/IF
Immunofluorescent analysis of Phospho-MAPK1/3 pThr202/Tyr204 (green) in HeLa cells either left untreated (left panel) or treated with 50nM PMA (right panel) for 10 minutes. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a Phospho-MAPK1/3 pThr2... View More
Product Details
PA1-4607
Applications
Tested Dilution
Publications
Product Specifications
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr202/Tyr204 of rat MAPK1/2 conjugated to KLH
Conjugate
Form
Liquid
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with bovine, canine, chicken, mouse, non-human primate, Xenopus and zebrafish based on 100% sequence homology.
In Western blot, this antibody detects an ~42-44 kDa protein representing Phospho-Thr202/Thr204 ERK/MAPK.
Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. Further optimization, including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody, may have to be performed for your high content assay.
Target Information
ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase and activates it as well. Once actived, Raf phosphorylates serine residue in 2 further kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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