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Invitrogen

Phospho-CaMKII alpha/beta (Thr286) Polyclonal Antibody

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View all (6) CaMKII alpha/beta antibodies

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Phospho-CaMKII alpha/beta (Thr286) Polyclonal Antibody

Product Details

PA1-4614

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
View 2 publications 2 publications

Immunohistochemistry (IHC)

1:1,000
-
Product Specifications

Species Reactivity

Human, Mouse, Rabbit, Rat

Published species

Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr286 of rat CAMK2A/B conjugated to KLH
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Amount

25 µg

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2259386

Product Specific Information

This antibody is predicted to react with human, mouse and Xenopus based on 100% sequence homology.

This antibody is specific for the ~50 kDa alpha-CaM Kinase II subunit and the ~60 kDa beta-CaM Kinase II subunit phosphorylated at Thr286 in Western blots of rat brain lysates. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.

Target Information

Ca2+/Calmodulin-Dependent Protein Kinase II (CaM Kinase II) is a multi-functional calcium and calmodulin-dependent protein kinase that mediates cellular response to a wide variety of intercellular signals (Kennedy, 1998; Schulman and Hanson, 1993). CaM Kinase II has been shown to regulate diverse cellular functions including synaptic plasticity, neurotransmitter synthesis and release, gene expression, ion channel function, carbohydrate metabolism, cytoskeletal function, and Ca2+-homeostasis (Gleason et al., 2003; Soderling, 2000; Hudmon and Schulman, 2002). Phosphorylation of Thr286 on the kinase produces an autonomously active form of CaM Kinase II (Meng et al., 2003; Picciotto et al., 1993). Autophosphorylation of Thr305 inhibits the activity CaM Kinase II. Phosphorylation at this site appears to reduce the association of CaM Kinase II with the PSD and reduces LTP and learning (Elgersma et al., 2002).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: alpha CaM kinase II; alpha-CaMKII; alpha-KAP; alternatively spliced; beta M CaM kinase II; brain type II Ca2+/calmodulin-dependent protein kinase beta subunit; Ca++/calmodulin-dependent protein kinase II beta subunit; Ca2+/calmodulin-dependent protein kinase II alpha; Calcium, calmodulin-dependent protein kinase II; calcium-calmodulin-dependent protein kinase II; calcium/calmodulin-dependent protein kinase; calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha; calcium/calmodulin-dependent protein kinase (CaM kinase) II beta; calcium/calmodulin-dependent protein kinase II alpha subunit; calcium/calmodulin-dependent protein kinase II alpha-B subunit; calcium/calmodulin-dependent protein kinase type II alpha chain; calcium/calmodulin-dependent protein kinase type II beta chain; CaM kinase II alpha subunit; CaM kinase II beta subunit; caM kinase II subunit alpha; caM kinase II subunit beta; CaM-kinase II alpha chain; CaM-kinase II beta chain; CaMK II; CaMK-II alpha subunit; caMK-II subunit alpha; caMK-II subunit beta; proline rich calmodulin-dependent protein kinase; protein kinase homolog; unnamed protein product

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Gene Aliases: CAM2; CAMK2; CAMKA; CAMKB; CaMKII; CaMKIIalpha; CaMKIIbeta; CaMKIINalpha; Ck2b; mKIAA0968; MRD53; MRD54; MRT63; PK2CDD; PKCCD

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UniProt ID: (Human) Q9UQM7, (Human) Q13554, (Mouse) P11798, (Rat) P11275, (Rat) P08413, (Mouse) P28652

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Entrez Gene ID: (Human) 815, (Rabbit) 100341507, (Human) 816, (Rabbit) 100342451, (Mouse) 12322, (Rat) 25400, (Rat) 24245, (Mouse) 12323

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Function(s)
protein kinase activity protein serine/threonine kinase activity calmodulin-dependent protein kinase activity protein binding calmodulin binding ATP binding glutamate receptor binding identical protein binding protein homodimerization activity protein serine kinase activity kinase activity GTPase activating protein binding protein kinase binding phospholipase binding structural constituent of postsynaptic actin cytoskeleton non-receptor serine/threonine protein kinase
Process(es)
G1/S transition of mitotic cell cycle response to ischemia calcium ion transport positive regulation of cardiac muscle cell apoptotic process cellular response to interferon-beta angiotensin-activated signaling pathway positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of JAK-STAT cascade regulation of neurotransmitter secretion regulation of neuronal synaptic plasticity positive regulation of calcium ion transport long-term synaptic potentiation dendritic spine development cellular response to interferon-gamma negative regulation of ferroptosis regulation of mitochondrial membrane permeability involved in apoptotic process regulation of protein localization to plasma membrane regulation of endocannabinoid signaling pathway regulation of neuron migration protein phosphorylation signal transduction positive regulation of neuron projection development regulation of skeletal muscle adaptation protein autophosphorylation regulation of long-term neuronal synaptic plasticity regulation of synapse structural plasticity regulation of calcium ion transport regulation of dendritic spine development positive regulation of dendritic spine morphogenesis positive regulation of synapse maturation JAK-STAT cascade peptidyl-serine phosphorylation ionotropic glutamate receptor signaling pathway dendrite morphogenesis regulation of neurotransmitter receptor localization to postsynaptic specialization membrane neurotransmitter receptor transport to plasma membrane peptidyl-threonine autophosphorylation inhibitory G-protein coupled receptor phosphorylation positive regulation of phospholipase activity regulation of synaptic transmission, cholinergic response to cadmium ion neuromuscular process controlling balance activation of meiosis involved in egg activation regulation of synapse maturation postsynaptic actin cytoskeleton organization hippocampal neuron apoptotic process cellular response to homocysteine response to psychosocial stress positive regulation of apoptotic signaling pathway
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