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Glucocorticoid Receptor Polyclonal Antibody
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FIGURE: 1 / 11
Glucocorticoid Receptor Antibody (PA1-510A) in ICC/IF
Immunofluorescence analysis of Glucocorticoid Receptor was performed using 70% confluent log phase A-549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Glucocorticoid Receptor Rabbit Polyclonal Antibody (Product # PA1-510A) at 1:250 dilution in 0.1% ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA1-510A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Reptile,
Rat
Published species
Human,
Mouse,
Rabbit,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic Peptide: A(150) P T E K E F P K T H S D V S S E Q Q H L K G Q T G(175)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
whole serum, PBS
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-510A detects glucocorticoid receptor (GR) from human, rat, and reptile tissues. This product detects both the unactivated and activated forms of the receptor.
PA1-510A has been successfully used in Western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunofluorescence, and gel shift procedures. By Western blot, this antibody detects an ~97 kDa protein representing GR from rat liver extract.
PA1-510A immunizing peptide corresponds to amino acid residues 150- 175 from human GR.
Target Information
Glucocorticoid Receptor (NR3C1) is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. Glucocorticoid Receptor can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Glucocorticoid Receptor is expressed in the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart. Alternate splicing, the use of at least three different promoters, and alternate translation initiation sites result in several transcript variants encoding the same protein or different isoforms, but the full-length nature of some variants has not been determined. Mutations in the Glucocorticoid Receptor gene are a cause of glucocorticoid resistance, or cortisol, resistance. Studies have shown that glucocorticoid receptor (GR) must be associated with a complex of chaperone proteins for ligand activation. GR binds to known steroids such as dexamethasone with nanomolar affinity
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: glucocorticoid receptor; glucocorticoid receptor 1; glucocorticoid receptor subtype P; GR; gr alpha; GR Kit; hGR DL-1; hGR NS-1; nuclear receptor subfamily 3 group C member 1 variant hGR-B(54); nuclear receptor subfamily 3 group C member 1 variant hGR-B(77); nuclear receptor subfamily 3 group C member 1 variant hGR-B(93); nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor); nuclear receptor; polymorphic form; OTTHUMP00000222854; OTTHUMP00000222858; putative upstream ORF.; Unknown; unnamed protein product
Gene Aliases: GCCR; GCR; GCRST; GR; GRL; Grl-1; Grl1
UniProt ID: (Human) P04150, (Rat) P06536, (Mouse) P06537
Entrez Gene ID: (Human) 2908, (Rat) 24413, (Mouse) 14815
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
core promoter sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
steroid hormone receptor activity
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
glucocorticoid receptor activity
steroid binding
protein binding
zinc ion binding
TBP-class protein binding
protein kinase binding
estrogen response element binding
identical protein binding
sequence-specific DNA binding
Hsp90 protein binding
steroid hormone binding
sequence-specific double-stranded DNA binding
promoter-specific chromatin binding
chromatin binding
double-stranded DNA binding
transcription coactivator activity
Hsp70 protein binding
receptor tyrosine kinase binding
heat shock protein binding
hormone binding
macromolecular complex binding
negative regulation of transcription from RNA polymerase II promoter
positive regulation of cytokine production
regulation of gluconeogenesis
chromatin remodeling
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
female pregnancy
circadian rhythm
glucocorticoid metabolic process
associative learning
response to wounding
gene expression
regulation of glucose metabolic process
microglia differentiation
positive regulation of glutamate secretion
response to activity
response to inactivity
muscle atrophy
lung development
adrenal gland development
intracellular steroid hormone receptor signaling pathway
negative regulation of synaptic plasticity
regulation of glucocorticoid biosynthetic process
nuclear receptor-mediated corticosteroid signaling pathway
response to insulin
Leydig cell differentiation
synaptic transmission, glutamatergic
regulation of cell proliferation
maternal behavior
glucocorticoid receptor signaling pathway
negative regulation of apoptotic process
negative regulation of vascular permeability
positive regulation of neuron apoptotic process
positive regulation of gene expression, epigenetic
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
response to arsenic-containing substance
response to mercury ion
astrocyte differentiation
response to glucocorticoid
response to corticosterone
response to cortisol
response to calcium ion
response to electrical stimulus
mammary gland duct morphogenesis
positive regulation of dendritic spine development
positive regulation of cell growth involved in cardiac muscle cell development
motor behavior
protein-containing complex assembly
cellular response to magnesium ion
cellular response to steroid hormone stimulus
cellular response to glucocorticoid stimulus
response to dexamethasone
cellular response to dexamethasone stimulus
cellular response to transforming growth factor beta stimulus
response to alcohol
neuroinflammatory response
negative regulation of long term synaptic depression
response to ketone
positive regulation of pri-miRNA transcription from RNA polymerase II promoter
regulation of nuclear receptor-mediated glucocorticoid signaling pathway
negative regulation of glucocorticoid receptor signaling pathway
positive regulation of glucocorticoid receptor signaling pathway
negative regulation of behavioral fear response
signal transduction
motor neuron apoptotic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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