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Invitrogen
CLOCK Polyclonal Antibody
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FIGURE: 1 / 18
CLOCK Antibody (PA1-520) in ICC/IF
Immunofluorescent analysis of CLOCK (green) showing staining in the nucleus and cytoplasm of U251 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CLOCK polyclonal antibody (Product # PA1-520) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLi... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA1-520
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Hamster,
Human,
Mouse,
Rat
Published species
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic Peptide: S(11) S I V D R D D S S I F D G L V E E D D(30) C
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
200 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-520 detects CLOCK from human, mouse, rat and hamster tissues.
PA1-520 has been successfully used in Western blot, ICC/IF and immunohistochemistry procedures. By Western blot, this antibody detects an ~100 kDa protein representing CLOCK from rat brain homogenate. Immunohistochemical staining of CLOCK in hamster brain with PA1-520 results in the staining of the superchiasmatic nucleus.
PA1-520 immunizing peptide corresponds to amino acid residues 11-30 of mouse CLOCK. This sequence is completely conserved between mouse and human CLOCK. PA1-520 immunizing peptide (Cat. # PEP-072) is available for use in neutralization and control experiments.
Target Information
This gene encodes a protein that belongs to the basic helix-loop-helix (bHLH) family of transcription factors. Polymorphisms within the encoded protein have been associated with circadian rhythm sleep disorders. A similar protein in mice is a circadian regulator that acts as a transcription factor and forms a heterodimer with aryl hydrocarbon receptor nuclear translocator-like to activate transcription of mouse period 1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: circadian locomoter output cycles kaput; circadian locomoter output cycles kaput protein; Circadian locomoter output cycles protein kaput; circadian locomotor output cycles kaput; class E basic helix-loop-helix protein 8; clock circadian regulator; clock homolog; KIAA0334; rCLOCK; transcription factor; unnamed protein product
Gene Aliases: 5330400M04Rik; bHLHe8; I79_020252; KAT13D
UniProt ID: (Human) O15516, (Rat) Q9WVS9, (Mouse) O08785
Entrez Gene ID: (Human) 9575, (Chinese hamster) 100770092, (Rat) 60447, (Mouse) 12753
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
histone acetyltransferase activity
protein binding
chromatin DNA binding
sequence-specific DNA binding
protein dimerization activity
peptide-lysine-N-acetyltransferase activity
E-box binding
sequence-specific double-stranded DNA binding
DNA damage checkpoint
chromatin remodeling
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
protein acetylation
spermatogenesis
female pregnancy
circadian rhythm
response to light stimulus
entrainment of circadian clock
gene expression
regulation of gene expression
circadian regulation of gene expression
regulation of hair cycle
regulation of circadian rhythm
positive regulation of circadian rhythm
proteasome-mediated ubiquitin-dependent protein catabolic process
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of inflammatory response
regulation of insulin secretion
response to redox state
cellular response to ionizing radiation
regulation of type B pancreatic cell development
negative regulation of glucocorticoid receptor signaling pathway
signal transduction
photoperiodism
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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