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Invitrogen
TEM8 Polyclonal Antibody
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FIGURE: 1 / 1
TEM8 Antibody (PA1-602) in WB
Western blot analysis was performed using membrane enriched extracts (30 µg) of Raji (Lane 1), U-87 MG (Lane 2), Hep G2 (Lane 3), Mouse Liver (Lane 4) and HeLa (Lane 5).The blots were probed with Anti-Anthrax Toxin Receptor Rabbit Polyclonal Antibody (Product # PA1-602, 1 in 1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4µg/mL 1:2500 dilution). A~ 65kDa band of Anthrax Toxin Receptor was observed across the cell lines tested. A ~... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA1-602
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic Peptide: L(92) M K L T E D R E Q I R Q G L E(107) C
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-602 detects Tem-8 from mouse samples.
PA1-602 has been successfully used in Western blot and immunohistochemical procedures. By Western blot, this antibody detects ~45 kDa, 60 kDa, 85 kDa proteins, representing Tem-8 from mouse tissue extracts.This antibody has also been used in immunohistochemical procedures (paraffin samples) using mouse tissues.
PA1-602 immunizing peptide corresponds to amino acid residues 92-107, within the extracellular domain, of human ATR/TEM8. This peptide (Cat. # PEP-253) is available for neutralization and control experiments.
Target Information
This gene encodes a type I transmembrane protein and is a tumor-specific endothelial marker that has been implicated in colorectal cancer. The encoded protein has been shown to also be a docking protein or receptor for Bacillus anthracis toxin, the causative agent of the disease, anthrax. The binding of the protective antigen (PA) component, of the tripartite anthrax toxin, to this receptor protein mediates delivery of toxin components to the cytosol of cells. Once inside the cell, the other two components of anthrax toxin, edema factor (EF) and lethal factor (LF) disrupt normal cellular processes. Three alternatively spliced variants that encode different protein isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 2310008J16Rik; 2810405N18Rik; anthrax toxin receptor; ATR; FLJ10601; FLJ11298; FLJ21776; mTEM8; Tem-8; tumor endothelial marker 8; unnamed protein product
Gene Aliases: 2310008J16Rik; 2810405N18Rik; Antrx1; ATR; GAPO; TEM8
UniProt ID: (Human) Q9H6X2, (Mouse) Q9CZ52
Entrez Gene ID: (Human) 84168, (Mouse) 69538
actin cytoskeleton organization
substrate adhesion-dependent cell spreading
positive regulation of epidermal growth factor receptor signaling pathway
blood vessel development
epidermal growth factor receptor signaling pathway
negative regulation of extracellular matrix assembly
positive regulation of metallopeptidase activity
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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