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Invitrogen
BACE1 Polyclonal Antibody
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FIGURE: 1 / 55
BACE1 Antibody (PA1-757) in ICC/IF
Immunofluorescent analysis of BACE1 (green) showing staining in the Golgi apparatus of Neuro-2a cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a BACE1 polyclonal antibody (Product # PA1-757) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark.... View More
Product Details
PA1-757
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues C(485) L R Q Q H D D F A D D I S L L K(501) of human beta-secretase.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-757 detects transfected beta-secretase 1.
PA1-757 has been successfully used in ELISA, ICC/IF, Western blot and immunoprecipitation applications. The molecular weight of BACE1 detected may vary depending on the lysate tested. This may be due to glycosylation differences in various cell lines.
The PA1-757 immunizing peptide (Cat. # PEP-093) is available for use in neutralization and control experiments.
Target Information
BACE1 (beta-secretase) is a key enzyme involved in the production of amyloid beta-peptides (Abeta) found in extracellular amyloid plaques of Alzheimer's disease (AD). In some cases early onset familial AD can be attributed to a "Swedish" mutation in the amyloid precursor protein (APP), which dramatically enhances the cleavage of this protein by BACE1. This and other genetic and pathological evidence has led to therapeutic approaches that have focused on the inhibition of BACE1 and other APP-cleaving enzymes, such as gamma-secretase stuff. APP is first cleaved by b-secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for g-secretase to generate the 4 kDa amyloid-b peptide, which is deposited in the brains of all sufferers of Alzheimer's disease. The long-sought b-secretase was recently identified by several groups independently and designated beta-site APP cleaving enzyme (BACE) and aspartyl protease 2 (Asp2). bACE/Asp2 is a novel transmembrane aspartic protease and colocalizes with APP.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: APP beta-secretase; asp 2; ASP2; Aspartyl protease 2; BACE; BACE 1; BACE-1B; BACE-1C; BACE-1D; BACE-I-432; BACEdelta7; Beta secretase; Beta-secretase 1; beta-site amyloid beta A4 precursor protein-cleaving enzyme; Beta-site amyloid precursor protein cleaving enzyme 1; Beta-site APP cleaving enzyme 1; beta-site APP processing enzyme; beta-site APP-cleaving enzyme; Memapsin; Memapsin-2; Memapsin2; membrane type aspartyl protease; Membrane-associated aspartic protease 2; transmembrane aspartic proteinase Asp2; unnamed protein product
Gene Aliases: ASP2; BACE; BACE1; C76936; HSPC104; KIAA1149
UniProt ID: (Human) P56817, (Mouse) P56818
Entrez Gene ID: (Human) 23621, (Mouse) 23821
proteolysis
membrane protein ectodomain proteolysis
response to lead ion
protein processing
beta-amyloid formation
amyloid precursor protein catabolic process
positive regulation of neuron apoptotic process
beta-amyloid metabolic process
modulation of synaptic transmission
detection of mechanical stimulus involved in sensory perception of pain
prepulse inhibition
cellular response to copper ion
cellular response to manganese ion
presynaptic modulation of chemical synaptic transmission
signaling receptor ligand precursor processing
cellular response to beta-amyloid
amyloid fibril formation
protein catabolic process
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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