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Invitrogen
PUMA alpha Polyclonal Antibody
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA1-84091
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide ARARQEGSSPEPVEG corresponding to the N-terminus of human PUMA alpha (aa 2-16).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Predicted to react with mouse based on sequence homology.
Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) is recommended for the staining of paraffin sections. A suggested positive control for this product is human breast carcinoma. In Western blot, this antibody detects a band at ~23kDa in K562 cell lysate.
Rabbit anti PUMA antibody recognizes the p53 - upregulated modulator of apoptosis, also known as Bcl-2 binding component 3, JFY-1 or Bcl-2.
Target Information
PUMA is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis. Antisense inhibition of PUMA expression reduced the apoptotic response to p53, and PUMA is likely to play a role in mediating p53-induced cell death through the cytochrome c/Apaf-1-dependent pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ42994; p53 up-regulated modulator of apoptosis; p53-induced BH3-only protein; p53-upregulated modulator of apoptosis; unnamed protein product
Gene Aliases: JFY-1; JFY1; PUMA
UniProt ID: (Human) Q96PG8
Entrez Gene ID: (Human) 27113
release of cytochrome c from mitochondria
apoptotic process
cellular response to DNA damage stimulus
determination of adult lifespan
positive regulation of protein complex assembly
response to endoplasmic reticulum stress
positive regulation of apoptotic process
positive regulation of neuron apoptotic process
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
positive regulation of thymocyte apoptotic process
cellular response to hypoxia
positive regulation of release of cytochrome c from mitochondria
apoptotic signaling pathway
intrinsic apoptotic signaling pathway
execution phase of apoptosis
positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway
positive regulation of IRE1-mediated unfolded protein response
positive regulation of intrinsic apoptotic signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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