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Invitrogen
PMCA2 ATPase Polyclonal Antibody
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FIGURE: 1 / 44
PMCA2 ATPase Antibody (PA1-915) in IHC (P)
Immunohistochemistry was performed on cancer biopsies of deparaffinized Human cervical carcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit po... View More
Product Details
PA1-915
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Pig,
Rat
Published species
Chicken,
Hamster,
Human,
Mouse,
Pig,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic Peptide: T(5) N S D F Y S K N Q R N E S S(19)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-915 detects calcium pump of the plasma membrane 2 (PMCA2 ATPase) from human and rat and porcine tissues and cells.
PA1-915 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~127 kDa protein and an ~133 kDa protein representing PMCA2a and PMCA2b ATPase, respectively, from rat brain microsomal fractions.
PA1-915 immunizing peptide corresponds to amino acid residues 5-19 from human PMCA2 ATPase protein. This sequence is completely conserved between human and rat PMCA2 ATPase.
Target Information
The calcium pump of the plasma membrane, termed PMCA ATPase, pumps calcium from the cytosol to the extracellular space. This membrane-bound enzyme is related to a number of other ATPases including the SERCA ATPase and the sodium/K+ pump. There are four different genes encoding PMCA ATPase and studies have revealed 20 isoforms of the pump generated by alternate splicing of the primary gene products. mRNA distribution studies show that gene products 1 and 4 are transcribed in most tissues, however, products 2 and 3 are more tissue specific. Transcription of the splicing variants has also been found to be tissue specific. In the pancreas, where insulin secretion is calcium dependent, the beta cells only express the 4b isoform, however the alpha and gamma cells express both 4a and 4b isoforms. Studies have also shown that different splice variants have different affinities for calcium and calmodulin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ATPase isoform 2 Na+K+ transporting beta polypeptide 2; ATPase isoform 2, Na+K+ transporting, beta polypeptide 2; ATPase, Ca++ transporting, plasma membrane 2; plasma membrane Ca(2+)-ATPase; plasma membrane Ca++-ATPase; plasma membrane Ca2+ pump 2; plasma membrane calcium ATPase; Plasma membrane calcium ATPase isoform 2; plasma membrane calcium pump; Plasma membrane calcium pump isoform 2; plasma membrane calcium-ATPase isoform 2 alternative splice variant a at splice site C; plasma membrane calcium-ATPase isoform 2, splice variant i; Plasma membrane calcium-transporting ATPase 2; PMCA2
Gene Aliases: ATP2B2; DFNA82; PMCA2; PMCA2a; PMCA2i
UniProt ID: (Human) Q01814, (Rat) P11506
Entrez Gene ID: (Human) 491, (Pig) 100625418, (Rat) 24215
nucleotide binding
calcium-transporting ATPase activity
calcium ion binding
protein binding
calmodulin binding
ATP binding
ATPase activity
PDZ domain binding
metal ion binding
P-type calcium transporter activity involved in regulation of postsynaptic cytosolic calcium ion concentration
protein C-terminus binding
calcium-dependent ATPase activity
acetylcholine receptor binding
glutamate receptor binding
primary active transporter
ion transport
calcium ion transport
sensory perception of sound
neuron differentiation
ion transmembrane transport
regulation of cytosolic calcium ion concentration
calcium ion transmembrane transport
regulation of postsynaptic cytosolic calcium ion concentration
regulation of cardiac conduction
cell morphogenesis
neural retina development
cellular calcium ion homeostasis
organelle organization
brain development
lactation
locomotory behavior
metabolic process
regulation of cell size
cerebellum development
cerebellar Purkinje cell layer morphogenesis
cerebellar Purkinje cell differentiation
cerebellar granule cell differentiation
locomotion
serotonin metabolic process
inner ear morphogenesis
otolith mineralization
cGMP metabolic process
regulation of synaptic plasticity
inner ear development
synapse organization
neuromuscular process controlling balance
detection of mechanical stimulus involved in sensory perception of sound
positive regulation of calcium ion transport
auditory receptor cell stereocilium organization
inner ear receptor cell differentiation
cochlea development
calcium ion export
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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