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          • Primary Antibodies ›
          • JNK1/JNK2 Antibodies

          Invitrogen

          Phospho-JNK1/JNK2 (Thr183, Tyr185) Polyclonal Antibody

          View all (13) JNK1/JNK2 antibodies

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          Cite Phospho-JNK1/JNK2 (Thr183, Tyr185) Polyclonal Antibody

          Additional Information:
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          Phospho-JNK1/JNK2 (Thr183, Tyr185) Polyclonal Antibody

          Product Details

          PA1-9593

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:2,000-1:4,000
          -

          Immunohistochemistry (IHC)

          1:500-1:2,000
          -

          Immunocytochemistry (ICC/IF)

          1:500-1:2,000
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Phosphorylated peptide (pT183-P-pY185) based on human JNK
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Amount

          15 µg

          Purification

          Affinity chromatography

          Storage buffer

          PBS

          Contains

          no preservative

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2235043

          Product Specific Information

          PA1-9593 detects Phospho-JNK/SAPK in human, mouse, and rat samples.

          PA1-9593 has been successfully used in Western blot, immunohistochemistry, and immunocytochemistry procedures. By Western blot, PA1-9593 detects a 46 kDa and 54 kDa protein representing Phospho-JNK/SAPK.

          PA1-9593 immunogen is a phosphorylated peptide (pT183-P-pY185) based on human JNK.

          Target Information

          C-Jun N-terminal Kinase (JNK) is also known as Mitogen-activated protein kinase (MAPK8). It belongs to the MAPK superfamily of stress-activated protein kinases. MAPKs are Serine-threonine protein kinases that are activated in response to a variety of extracellular stimuli, and mediate signal transduction from the cell surface to the nucleus. JNK is involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. JNK pathways are activated by stress and inflammatory signals. JNK is expressed as ten different isoforms due to differential mRNA splicing. The predominant forms are JNK1 and JNK2.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: C-jun kinase; c-Jun kinase 2; c-Jun N-terminal kinase 1; c-Jun N-terminal kinase 2; c-jun NH2-terminal kinase; JNK 55; JNK/SAPK alpha; JNK1 beta1 protein kinase; Jun kinase; JUN N-terminal kinase; MAP kinase 8; MAP kinase 9; MAPK; MAPK 8; MAPK 9; MAPK8; MAPK9; OTTHUMP00000019552; p54 gamma; p54-alpha; Phospho-JNK; PRKM; protein kinase JNK1; protein kinase mitogen-activated 8; protein kinase, mitogen-activated 9; SAPK 1; SAPK gamma; SAPK-alpha; stress activated protein kinase alpha II; stress-activated protein kinase 1; stress-activated protein kinase 1a; stress-activated protein kinase 1c; stress-activated protein kinase JNK1; stress-activated protein kinase JNK2; unnamed protein product

          View more View less

          Gene Aliases: JNK; JNK-46; JNK-55; JNK1; JNK1A2; JNK2; JNK21B1/2; JNK2A; JNK2ALPHA; JNK2B; JNK2BETA; p54a; p54aSAPK; PRKM8; PRKM9; SAPK; SAPK1; SAPK1a; SAPK1c

          View more View less

          UniProt ID: (Human) P45983, (Human) P45984, (Mouse) Q91Y86, (Rat) P49185, (Mouse) Q9WTU6, (Rat) P49186

          View more View less

          Entrez Gene ID: (Human) 5599, (Human) 5601, (Mouse) 26419, (Rat) 116554, (Mouse) 26420, (Rat) 50658

          View more View less

          Function(s)
          protein kinase activity protein serine/threonine kinase activity JUN kinase activity MAP kinase activity protein binding ATP binding enzyme binding protein phosphatase binding histone deacetylase regulator activity histone deacetylase binding protein serine kinase activity protein serine/threonine kinase binding protein serine/threonine/tyrosine kinase activity kinase activity kinesin binding mitogen-activated protein kinase kinase kinase binding
          Process(es)
          MAPK cascade protein phosphorylation response to oxidative stress JNK cascade JUN phosphorylation response to UV response to mechanical stimulus positive regulation of gene expression regulation of macroautophagy peptidyl-serine phosphorylation peptidyl-threonine phosphorylation positive regulation of cyclase activity positive regulation of cell killing negative regulation of protein binding regulation of protein localization cellular response to amino acid starvation cellular response to oxidative stress cellular response to reactive oxygen species Fc-epsilon receptor signaling pathway regulation of circadian rhythm positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of protein metabolic process stress-activated MAPK cascade cellular response to lipopolysaccharide cellular response to mechanical stimulus cellular senescence energy homeostasis positive regulation of NLRP3 inflammasome complex assembly positive regulation of establishment of protein localization to mitochondrion release of cytochrome c from mitochondria positive regulation of protein phosphorylation positive regulation of macrophage derived foam cell differentiation neuron projection development positive regulation of prostaglandin biosynthetic process regulation of protein ubiquitination positive regulation of protein ubiquitination positive regulation of prostaglandin secretion positive regulation of proteasomal ubiquitin-dependent protein catabolic process positive regulation of chemokine production cellular response to UV regulation of mRNA stability positive regulation of cell differentiation positive regulation of transcription, DNA-templated regulation of JNK cascade response to cadmium ion modulation of synaptic transmission mRNA destabilization protein localization to tricellular tight junction positive regulation of podosome assembly inflammatory response to wounding extrinsic apoptotic signaling pathway positive regulation of cytokine production involved in inflammatory response positive regulation of apoptotic signaling pathway ossification neuron migration positive regulation of neuroblast proliferation double-strand break repair regulation of transcription, DNA-templated integrin-mediated signaling pathway neuromuscular junction development regulation of gene expression positive regulation of cardiac muscle cell apoptotic process positive regulation of cell migration positive regulation of microtubule polymerization positive regulation of glial cell apoptotic process ERBB signaling pathway response to hydrogen peroxide positive regulation of neuron apoptotic process NLRP3 inflammasome complex assembly regulation of neuron differentiation positive regulation of DNA replication regulation of centrosome cycle determination of dorsal identity dendrite morphogenesis SMAD protein signal transduction protein poly-ADP-ribosylation cellular response to hydrogen peroxide cellular response to nitric oxide cellular response to oxygen-glucose deprivation type B pancreatic cell apoptotic process neuronal stem cell population maintenance programmed necrotic cell death regulation of dense core granule transport protein localization to site of double-strand break positive regulation of determination of dorsal identity positive regulation of neuron migration response to xenobiotic stimulus response to toxic substance response to amine neuron development protein localization to mitochondrion cellular response to interleukin-1 cellular response to tumor necrosis factor cellular response to growth factor stimulus cellular hyperosmotic response cellular response to beta-amyloid response to tetrachloromethane response to nerve growth factor glial cell apoptotic process response to steroid hormone
          It has to be done as per old AB suggested Products section.

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