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Invitrogen

MRE11 Polyclonal Antibody

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MRE11 Polyclonal Antibody

Product Details

PA3-16527

Applications
Tested Dilution
Publications

Western Blot (WB)

1:5,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:500
-

Immunocytochemistry (ICC/IF)

1:200
View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-Dependent
-

Immunoprecipitation (IP)

3 µL
-

ChIP assay (ChIP)

Assay-Dependent
-
Product Specifications

Species Reactivity

Chicken, Hamster, Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Full length human Mre11 protein.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2145113

Product Specific Information

This Mre11 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections (PMID 21279473) Immunoprecipitation and Western Blot.

In WB, a band can be seen at ~81 kDa.

For ICC/IF, this antibody has been used with methanol-fixed IMR90 primary human fibroblasts. For IP, the suggested working dilution is 3 µL for immunoprecipitation of 3X10^6 cells.

Co-IP application has been reported by Ching et al 2012 (PMID: 22190719).

Suggested positive control: antigen standard for MRE11A (transient overexpression lysate), Hela whole cell extract, Hela whole cell lysate.

Predicted cross-reactivity based on sequence identity: Gorilla/Chimpanzee (100%), Gibbon (99%), Marmoset (96%), Canine/Feline/Panda (94%), Equine/Bovine/Bat (92%).

Target Information

MRE11 (double-strand break repair protein MRE11) is a component of the MRN complex. This complex plays a central role in double-strand break repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-stranded endonuclease activity and double-strand-specific 3'-5' exonuclease activity which are provided by MRE11. RAD50 is required to bind DNA ends and hold them in close proximity. Mutations in the gene could result in ataxia-telangiectasia-like disorder 1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: AT-like disease; DNA recombination and repair protein; Double-strand break repair protein MRE11; Double-strand break repair protein MRE11A; endo/exonuclease Mre11; hMRE11B; Mre11 homolog; Meiotic recombination 11 homolog 1; Meiotic recombination 11 homolog A; mmMRE11A; MRE11 double strand break repair nuclease A; MRE11 homolog 1; MRE11 homolog A; MRE11 homolog A, double strand break repair nuclease; MRE11 homolog, double strand break repair nuclease A; MRE11 meiotic recombination 11 homolog A; MRE11 meiotic recombination 11 homolog A (S. cerevisiae); MRE11 meiotic recombination 11 homolog A (S. cerevisiae) isoform MRE11A_1; MRE11 meiotic recombination 11 homolog A (S. cerevisiae) isoform MRE11A_2; MRE11 meiotic recombination 11-like protein A; unnamed protein product

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Gene Aliases: ATLD; HNGS1; MRE11; MRE11A; MRE11B

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UniProt ID: (Human) P49959, (Chicken) Q9IAM7, (Rat) Q9JIM0, (Mouse) Q61216

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Entrez Gene ID: (Human) 4361, (Chicken) 395555, (Rat) 64046, (Chinese hamster) 100750877, (Mouse) 17535

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Function(s)
single-stranded DNA endodeoxyribonuclease activity DNA binding DNA helicase activity double-stranded DNA binding nuclease activity endonuclease activity endodeoxyribonuclease activity exonuclease activity protein binding 3'-5'-exodeoxyribonuclease activity 3'-5' exonuclease activity hydrolase activity manganese ion binding identical protein binding cadherin binding 5'-3' exonuclease activity ATP-dependent DNA helicase activity protein C-terminus binding
Process(es)
telomere maintenance base-excision repair nucleotide-excision repair double-strand break repair cellular response to DNA damage stimulus sister chromatid cohesion mitotic G2 DNA damage checkpoint synapsis heart development cell proliferation intra-S DNA damage checkpoint positive regulation of protein autophosphorylation DNA duplex unwinding negative regulation of DNA endoreduplication positive regulation of kinase activity negative regulation of viral entry into host cell chromosome organization meiotic cell cycle nucleic acid phosphodiester bond hydrolysis regulation of mitotic recombination double-strand break repair via homologous recombination DNA double-strand break processing DNA repair double-strand break repair via nonhomologous end joining DNA recombination telomere maintenance via telomerase reciprocal meiotic recombination telomeric 3' overhang formation positive regulation of telomere maintenance reciprocal DNA recombination meiotic DNA double-strand break formation negative regulation of apoptotic process mitotic G2/M transition checkpoint R-loop processing mitochondrial double-strand break repair via homologous recombination DNA strand resection involved in replication fork processing positive regulation of double-strand break repair negative regulation of double-strand break repair via nonhomologous end joining DNA metabolic process
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