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Invitrogen

PPAR gamma Polyclonal Antibody

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23 Published Figures
40 References
View all (34) PPAR gamma antibodies

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PPAR gamma Polyclonal Antibody

Product Details

PA3-821A

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,500
View 21 publications 21 publications

Immunohistochemistry (IHC)

-
View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

-
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:250
View 4 publications 4 publications

Flow Cytometry (Flow)

-
View 3 publications 3 publications

Immunoprecipitation (IP)

-
View 2 publications 2 publications

ChIP assay (ChIP)

-
View 3 publications 3 publications

Proximity Ligation Assay (PLA) (PLA)

-
View 1 publication 1 publication

Gel Shift (EMSA)

-
View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Pig, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues M(284) M G E D K I K F K H I T P L(298) of mouse PPAR gamma 2.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2166056

Product Specific Information

PA3-821A detects peroxisome proliferator activated receptor (PPAR) gamma from human and mouse samples. This antibody is a reproduction of PA3-821, using the same immunizing peptide and new host rabbits.

PA3-821A has been successfully used in IF and Western blot procedures. Previous batches of this antibody have been used in gel shift, immunocytochemistry, and immunofluoresence procedures. By Western blot, this antibody detects a 57 kDa protein representing PPAR gamma 2 from differentiated mouse 3T3-L1 cell lysate. Immunolocalization of previous batches gives predominant nuclear staining.

The PA3-821A immunizing peptide corresponds to amino acid residues 284-298 from mouse PPAR gamma 2. This sequence is completely conserved in PPAR gamma 1, but exhibits no significant homology with PPAR alpha or NUC1. This sequence is completely conserved in mouse, human, and rat.

Target Information

This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: HUMPPARG; ligand-activated nuclear transcription factor; ligand-activated transcription factor; nuclear receptor; nuclear receptor family member; Pparg; nuclear receptor subfamily 1 group C member 3; OTTHUMP00000185032; OTTHUMP00000185036; peroxisome proliferator; peroxisome proliferator activated receptor; peroxisome proliferator activated receptor gamma 2; peroxisome proliferator activated receptor gamma 4; peroxisome proliferator activator receptor, gamma; peroxisome proliferator-activated receptor-gamma 5; peroxisome proliferator-activated receptor-gamma splicing; PPAR gamma; PPAR-gamma; PPAR-gamma-1a; PPAR-gamma-1b; Pparg; PPARy; unnamed protein product

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Gene Aliases: CIMT1; FPLD3; GLM1; NR1C3; PPAR-gamma; PPAR-gamma2; PPARG1; PPARG2; PPARG5; PPARgamma; PPARgamma2

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UniProt ID: (Human) P37231, (Rat) O88275, (Mouse) P37238

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Entrez Gene ID: (Human) 5468, (Rat) 25664, (Mouse) 19016

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Function(s)
transcription regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcription cofactor binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding nucleic acid binding DNA binding chromatin binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding prostaglandin receptor activity protein binding zinc ion binding enzyme binding peptide binding identical protein binding sequence-specific DNA binding retinoid X receptor binding arachidonic acid binding DBD domain binding LBD domain binding WW domain binding alpha-actinin binding R-SMAD binding E-box binding STAT family protein binding DNA-binding transcription factor binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding transcription coactivator binding protein phosphatase binding estrogen receptor binding promoter-specific chromatin binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter negative regulation of cytokine production placenta development response to dietary excess diet induced thermogenesis wound healing involved in inflammatory response negative regulation of acute inflammatory response regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter fatty acid metabolic process response to stress heart development regulation of blood pressure negative regulation of cell proliferation response to xenobiotic stimulus response to light stimulus response to mechanical stimulus hormone-mediated signaling pathway regulation of gene expression negative regulation of cardiac muscle hypertrophy positive regulation of gene expression negative regulation of gene expression negative regulation of macrophage derived foam cell differentiation positive regulation of cholesterol efflux negative regulation of cholesterol storage negative regulation of lipid storage negative regulation of smooth muscle cell migration long-chain fatty acid transport negative regulation of angiogenesis regulation of lipid metabolic process fatty acid oxidation cell differentiation monocyte differentiation negative regulation of cell growth BMP signaling pathway negative regulation of transforming growth factor beta receptor signaling pathway negative regulation of BMP signaling pathway intracellular receptor signaling pathway epithelial cell differentiation response to caffeine organ regeneration positive regulation of prostaglandin biosynthetic process response to nutrient levels response to food positive regulation of cholesterol transport positive regulation of intracellular cholesterol transport cellular response to insulin stimulus cellular response to hormone stimulus negative regulation of collagen biosynthetic process response to vitamin A response to lipid peroxisome proliferator activated receptor signaling pathway response to immobilization stress cellular response to platelet-derived growth factor stimulus glucose homeostasis response to starvation regulation of circadian rhythm mRNA transcription from RNA polymerase II promoter positive regulation of apoptotic process negative regulation of MAPK cascade negative regulation of blood vessel endothelial cell migration response to estrogen cell fate commitment fat cell differentiation regulation of fat cell differentiation positive regulation of fat cell differentiation negative regulation of osteoblast differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of fatty acid metabolic process positive regulation of transcription from RNA polymerase II promoter positive regulation of fatty acid oxidation regulation of hormone secretion retinoic acid receptor signaling pathway cell maturation negative regulation of smooth muscle cell proliferation positive regulation of oligodendrocyte differentiation positive regulation of protein secretion negative regulation of inflammatory response white fat cell differentiation brown fat cell differentiation positive regulation of phagocytosis, engulfment negative regulation of interferon-gamma-mediated signaling pathway positive regulation of SMAD protein import into nucleus negative regulation of SMAD protein import into nucleus positive regulation of adiponectin secretion response to linoleic acid cellular response to lithium ion cellular response to retinoic acid cellular response to vitamin E cellular response to prostaglandin stimulus cellular response to prostaglandin E stimulus cellular response to low-density lipoprotein particle stimulus cellular response to hyperoxia cellular response to hypoxia negative regulation of mitochondrial fission negative regulation of peptide hormone secretion cold-induced thermogenesis positive regulation of lipoprotein transport beige fat cell differentiation negative regulation of cellular response to insulin stimulus negative regulation of extracellular matrix assembly negative regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of pri-miRNA transcription from RNA polymerase II promoter regulation of protein localization to plasma membrane negative regulation of cardiac muscle hypertrophy in response to stress negative regulation of cellular response to transforming growth factor beta stimulus positive regulation of adipose tissue development negative regulation of connective tissue replacement involved in inflammatory response wound healing negative regulation of vascular smooth muscle cell proliferation negative regulation of receptor signaling pathway via STAT positive regulation of vascular associated smooth muscle cell apoptotic process negative regulation of vascular endothelial cell proliferation negative regulation of pancreatic stellate cell proliferation positive regulation of apoptotic signaling pathway G-protein coupled receptor signaling pathway response to nutrient innate immune response positive regulation of lipid metabolic process regulation of transport negative regulation of multicellular organismal process lipid homeostasis nuclear receptor-mediated signaling pathway regulation of cellular response to insulin stimulus inflammatory response
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