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Invitrogen
MELK Polyclonal Antibody
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Product Details
PA5-110824
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Protein corresonding to Human MELK. Recombinant protein control fragment (Product #RP-89807).
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: DIKSNNWSLE DVTASDKNYV AGLIDYDWCE DDLSTGAATP RTSQFTKYWT ESNGVESKSL TPALCRTPAN KLKNKENVYT PKSAVKNEEY FMFPEPKTPV NKNQHKREIL TTPNRYTTPS KARNQCLKET PIKIPVNSTG TD
Highest antigen sequence indentity to the following orthologs: Rat - 22%, Mouse - 58%.
Target Information
MELK is a serine/threonione kinase which is related to the AMPK family that has been linked to the cell cycle. MELK has also been shown to be overexpressed in cancer and has been identified has a potential target for cancer therapeutics. MELK has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation and carcinogenesis. Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14, possibly leading to affect mammary carcinogenesis by mediating inhibition of the pro-apoptotic function of BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hematopoiesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: hMELK; hPK38; Maternal embryonic leucine zipper kinase; OTTHUMP00000046113; pEg3 kinase; Protein kinase Eg3; Protein kinase PK38; RP11-8N6.1; Tyrosine-protein kinase MELK
Gene Aliases: HPK38; KIAA0175; MELK
UniProt ID: (Human) Q14680
Entrez Gene ID: (Human) 9833
G2/M transition of mitotic cell cycle
apoptotic process
cell proliferation
intrinsic apoptotic signaling pathway in response to oxidative stress
peptidyl-tyrosine phosphorylation
hemopoiesis
intracellular signal transduction
positive regulation of apoptotic process
protein autophosphorylation
neural precursor cell proliferation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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