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Invitrogen
IRE1 alpha Polyclonal Antibody
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Product Details
PA5-110946
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Protein corresonding to Human IRE1 alpha. Recombinant protein control fragment (Product #RP-90501).
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: VTIGDKGECV ITPSTDVKFD PGLKSKNKLN YLRNYWLLIG HHETPLSAST KMLERFPNNL PKHRENVIPA DSEKKSFEEV INLVDQTSEN APTTVSRDVE EKPAHAPARP EAPVDSMLKD MATIILSTFL LIGWVA
Highest antigen sequence indentity to the following orthologs: Rat - 91%, Mouse - 90%.
Target Information
ERN1 serine/threonine kinase or endoplasmic reticulum to nucleus signaling 1 is the ER to nucleus signaling 1 protein, a human homologue of the yeast Ire1 gene product which possesses intrinsic kinase activity and an endoribonuclease activity and it is important in endoplasmic reticulum-based stress signals. ERN1 controls IRE1 proteolysis in mammalian cells. ERN1 has a critical function in extraembryonic cells that is essential for fetal viability. Specifically, IRE1 acts as the sensor of unfolded proteins in the ER that initiates transmittance of the unfolded protein signal from the ER to the nucleus by splicing XBP1 mRNA converting it into a potent unfolded-protein response transcriptional activator. Null mutants are viable, but IRE1 is essential for viability under stress conditions that cause unfolded proteins to accumulate in the ER. Depletion of IRE1p through the expression of a dominant negative form of IRE1p has no effect on transfected cells, but cell death via apoptosis occurs under stress conditions that cause unfolded proteins to accumulate in the ER.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Endoplasmic reticulum-to-nucleus signaling 1; ER to nucleus signalling 1; FLJ30999; hIRE1p; inositol-requiring 1; inositol-requiring enzyme 1; Inositol-requiring protein 1; Ire1-alpha; IRE1a; IRE1a antibody; Ire1p; IRE1p antibody; MGC163277; MGC163279; protein kinase/endoribonuclease; Serine/threonine-protein kinase/endoribonuclease IRE1; unnamed protein product
Gene Aliases: ERN1; hIRE1p; IRE1; IRE1a; IRE1P
UniProt ID: (Human) O75460
Entrez Gene ID: (Human) 2081
nucleotide binding
magnesium ion binding
catalytic activity
endonuclease activity
endoribonuclease activity
ribonuclease activity
protein kinase activity
protein serine/threonine kinase activity
platelet-derived growth factor receptor binding
protein binding
ATP binding
kinase activity
transferase activity
hydrolase activity
enzyme binding
Hsp70 protein binding
identical protein binding
protein homodimerization activity
ADP binding
metal ion binding
unfolded protein binding
Hsp90 protein binding
protein serine kinase activity
endothelial cell proliferation
mRNA processing
RNA catabolic process
mRNA catabolic process
protein phosphorylation
apoptotic process
response to unfolded protein
negative regulation of gene expression
regulation of macroautophagy
endoplasmic reticulum unfolded protein response
positive regulation of RNA splicing
cellular response to unfolded protein
response to endoplasmic reticulum stress
cellular response to vascular endothelial growth factor stimulus
IRE1-mediated unfolded protein response
positive regulation of JNK cascade
mRNA splicing, via endonucleolytic cleavage and ligation
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
cellular response to hydrogen peroxide
cellular response to glucose stimulus
positive regulation of endoplasmic reticulum unfolded protein response
insulin metabolic process
positive regulation of vascular smooth muscle cell proliferation
positive regulation of response to endoplasmic reticulum stress
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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