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Invitrogen
DNA ligase IV Polyclonal Antibody
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Product Details
PA5-111451
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Protein corresonding to Human DNA ligase IV. Recombinant protein control fragment (Product #RP-108420).
Conjugate
Form
Liquid
Concentration
0.4 mg/mL
Amount
40 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: PQLERERMAY GIKETMLAKL YIELLNLPRD GKDALKLLNY RTPTGTHGDA GDFAMIAYFV LKPRCLQKGS LTIQQVNDLL DSIASNNSAK RKDLIKKSLL QLITQSSALE QKWLIRMIIK DLKLGVSQQT IFSVFHNDAA ELHNV
Highest antigen sequence indentity to the following orthologs: Rat - 88%, Mouse - 90%.
Target Information
The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA joinase; DNA ligase 4; DNA ligase IV; DNA repair enzyme; ligase IV, DNA, ATP-dependent; Polydeoxyribonucleotide synthase [ATP] 4; polynucleotide ligase; sealase; unnamed protein product
Gene Aliases: LIG4; LIG4S
UniProt ID: (Human) P49917
Entrez Gene ID: (Human) 3981
single strand break repair
in utero embryonic development
pro-B cell differentiation
DNA repair
base-excision repair
nucleotide-excision repair, DNA gap filling
double-strand break repair
double-strand break repair via nonhomologous end joining
DNA recombination
cellular response to DNA damage stimulus
central nervous system development
cell proliferation
response to X-ray
response to ionizing radiation
response to gamma radiation
neurogenesis
T cell differentiation in thymus
V(D)J recombination
immunoglobulin V(D)J recombination
T cell receptor V(D)J recombination
somatic stem cell population maintenance
negative regulation of neuron apoptotic process
isotype switching
fibroblast proliferation
positive regulation of fibroblast proliferation
positive regulation of neurogenesis
chromosome organization
cell division
neuron apoptotic process
cellular response to lithium ion
cellular response to ionizing radiation
DNA biosynthetic process
stem cell proliferation
establishment of integrated proviral latency
double-strand break repair via classical nonhomologous end joining
DN2 thymocyte differentiation
positive regulation of chromosome organization
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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