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Invitrogen
Nop25 Polyclonal Antibody
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Product Details
PA5-51958
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human Nop25. Recombinant protein control fragment (Product #RP-91493).
Conjugate
Form
Liquid
Concentration
0.3 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: HQEYLKMLAE REEALEEADE LDRLVTAKTE SVQYDHPNHT VTVTTISDLD LSGARLLGLT PPEGGAGDRS EEEASSTEKP TKALPRKSRD PLLSQRISSL TASLHAHSRK KVKRKHPRRA QDSKKPPRAP RTSKAQRRRL TGKAR
Highest antigen sequence identity to the following orthologs: Mouse - 88%, Rat - 86%.
Target Information
Nop25 is a nucleolar protein predominantly involved in the architecture and function of the nucleolus, which is the cellular hub for ribosomal RNA (rRNA) synthesis and ribosome assembly. It is identified as a putative RNA-binding protein and plays a crucial role in rRNA transcription. Nop25 has been characterized through molecular cloning, showing its ability to bind single-stranded nucleic acids directly. Experimental studies, such as those involving mouse embryogenesis, have utilized techniques like RT-PCR to analyze Nop25's gene expression across developmental stages. Moreover, research on the protein's structure has unveiled its nuclear and nucleolar targeting sequences, vital for its localization to the nucleolus. Treatment of cells with RNase A has illustrated the dissociation of Nop25 from the nucleolus, further highlighting its RNA-binding capabilities. This protein's functional importance is underscored by its role in maintaining nucleolar architecture, as demonstrated by experiments where overexpression of Nop25's N-terminal amino acids disrupted nucleolar integrity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Nucleolar protein 12
Gene Aliases: dJ37E16.7; NOL12; Nop25
UniProt ID: (Human) Q9UGY1
Entrez Gene ID: (Human) 79159
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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