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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • HCE Antibodies

          Invitrogen

          HCE Polyclonal Antibody

          View all (7) HCE antibodies

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          Cite HCE Polyclonal Antibody

          • Antibody Testing Data (4)
          HCE Antibody in Immunocytochemistry (ICC/IF)
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          HCE Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 4

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          HCE Antibody (PA5-51999) in ICC/IF

          Immunofluorescent staining of HCE in human cell line RH-30 using a HCE Polyclonal Antibody (Product # PA5-51999) shows localization to nucleoplasm. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          HCE Antibody in Immunocytochemistry (ICC/IF)
          HCE Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          HCE Antibody in Western Blot (WB)
          HCE Antibody in Western Blot (WB)
          HCE Polyclonal Antibody

          Product Details

          PA5-51999

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.04-0.4 µg/mL
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:20-1:50
          -

          Immunocytochemistry (ICC/IF)

          0.25-2 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant protein corresponding to Human HCE. Recombinant protein control fragment (Product #RP-102092).
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.1 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.2, with 40% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2642332

          Product Specific Information

          Immunogen sequence: IKLLDLKPYK VSWKADGTRY MMLIDGTNEV FMIDRDNSVF HVSNLEFPFR KDLRMHLSNT LLDGEMIIDR VNGQAVPRYL IYDIIKFNSQ PVGDCDFNVR LQCIEREIIS PRHEKMKTGL IDKTQEP

          Highest antigen sequence identity to the following orthologs: Mouse - 95%, Rat - 94%.

          Target Information

          Bifunctional mRNA-capping enzyme exhibiting RNA 5'-triphosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. Catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the gmp moiety of GTP to the 5'-diphosphate terminus.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

          Have you cited this product in a publication?
          Let us know so we can reference it here.
          Cite this product

          Bioinformatics

          Protein Aliases: HCAP1; HCE; MCE1; mRNA-capping enzyme; RP3-488C13.1

          View more View less

          Gene Aliases: AU020997; CAP1A; hCAP; HCE; HCE1; MCE1; RNGTT

          View more View less

          UniProt ID: (Human) O60942, (Mouse) O55236

          View more View less

          Entrez Gene ID: (Human) 8732, (Mouse) 24018, (Rat) 313131

          View more View less

          Function(s)
          mRNA guanylyltransferase activity polynucleotide 5'-phosphatase activity protein tyrosine phosphatase activity GTP binding protein tyrosine/serine/threonine phosphatase activity RNA guanylyltransferase activity triphosphatase activity molecular_function mRNA capping factor
          Process(es)
          transcription from RNA polymerase II promoter 7-methylguanosine mRNA capping RNA processing viral process peptidyl-tyrosine dephosphorylation polynucleotide 5' dephosphorylation biological_process
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

          Learn more
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          We're here to help

          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

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