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Invitrogen
SMARCB1 Polyclonal Antibody
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FIGURE: 1 / 12
SMARCB1 Antibody (PA5-53932) in ICC/IF
Immunofluorescence analysis of SMARCB1 was performed using HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with SMARCB1 Rabbit Polyclonal Antibody (Product # PA5-53932) at 1:200 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731) at a dilution of 1:2... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-53932
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human SMARCB1. Recombinant protein control fragment (Product #RP-92346).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.1 mg/mL
Amount
10 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: EKENSPEKFA LKLCSELGLG GEFVTTIAYS IRGQLSWHQK TYAFSENPLP TVEIAIRNTG DADQWCPLLE TLTDAEMEKK IRDQDRNTRR MRRLANTA
Highest antigen sequence identity to the following orthologs: Mouse - 100%, Rat - 100%.
Target Information
The SWI-SNF complex is involved in the activation of transcription via the remodeling of nucleosome structure in an ATP-dependent manner. Brm (also designated SNF2alpha) and Brg-1 (also designated SNF2beta) are the ATPase subunits of the mammalian SWI-SNF complex. Brm, Brg-1, Ini1 (integrase interactor 1, also designated SNF5), BAF155 (also designated SRG3) and BAF170 are thought to comprise the functional core of the SWI-SNF complex. Addition of Ini1, BAF155 and BAF170 to Brg-1 appears to increase remodeling activity. Other complex subunits are thought to play regulatory roles. hSNF2L and hSNF2H both appear to be homologs of Drosophila ISWI, a Brm related ATPase that is present in chromatin remodeling complexes other than SWI/SNF, including the NURF (nucleosome remodeling factor).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BRG1-associated factor 47; BRG1-associated factor 47 (BAF47); hSNF5; Ini1; Ini1b; INI1D; INI1E; INI1F; integrase interactor 1 protein; malignant rhabdoid tumor suppressor; mSNF5; protein phosphatase 1, regulatory subunit 144; SMARCB1; SNF5 homolog; Sucrose nonfermenting yeast homolog like 1; sucrose nonfermenting, yeast, homolog-like 1; SWI/SNF related matrix-associated actin dependent regulator of chromatin, subfamily b, member 1; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1; SWI/SNF-related matrix-associated protein; SWI10; Transcription factor TYE4; Transcription regulatory protein SNF5; TYE4; unnamed protein product
Gene Aliases: BAF47; CSS3; hSNFS; INI-1; INI1; MRD15; PPP1R144; RDT; RTPS1; Sfh1p; SNF5; SNF5/INI1; SNF5L1; Snr1; SWNTS1
UniProt ID: (Human) Q12824, (Mouse) Q9Z0H3
Entrez Gene ID: (Human) 6598, (Mouse) 20587, (Rat) 361825
RNA polymerase I preinitiation complex assembly
chromatin remodeling
regulation of transcription from RNA polymerase II promoter
DNA integration
regulation of mitotic metaphase/anaphase transition
single stranded viral RNA replication via double stranded DNA intermediate
positive regulation by host of viral transcription
positive regulation of T cell differentiation
positive regulation of cell differentiation
positive regulation of myoblast differentiation
positive regulation of gene expression, epigenetic
positive regulation of transcription from RNA polymerase II promoter
regulation of G0 to G1 transition
positive regulation of transcription of nuclear large rRNA transcript from RNA polymerase I promoter
positive regulation of stem cell population maintenance
positive regulation of glucose mediated signaling pathway
regulation of G1/S transition of mitotic cell cycle
positive regulation of double-strand break repair
regulation of nucleotide-excision repair
blastocyst development
blastocyst hatching
nucleosome disassembly
negative regulation of cell proliferation
gene expression
cell differentiation
protein-containing complex assembly
biological_process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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