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Invitrogen
Perilipin 1 Polyclonal Antibody
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FIGURE: 1 / 8
Perilipin 1 Antibody (PA5-55046) in ICC/IF
Immunofluorescence analysis of Perilipin 1 was performed using 100% confluent log phase differentiated 3T3-L1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Perilipin 1 Polyclonal Antibody (Product # PA5-55046) at 1:100 dilution in 0.1% BSA, incu... View More
Product Details
PA5-55046
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human Perilipin. Recombinant protein control fragment (Product #RP-88973).
Conjugate
Form
Liquid
Concentration
0.10 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: VCNAYEKGVQ SASSLAAWSM EPVVRRLSTQ FTAANELACR GLDHLEEKIP ALQYPPEKIA SELKDTISTR LRSARNSISV PIASTSDKVL GAALAGCELA WGVARDTAEF AANTRAGRLA SGGADLALGS IEKVVE
Highest antigen sequence identity to the following orthologs: Mouse - 93%, Rat - 93%.
Target Information
Adipose tissue is an energy reserve in animals and is strictly regulated in nondomestic species. Adipose cells produce and secrete numerous physiologically important proteins, such as lipoprotein lipase (LPL), leptin, adipocyte complement related protein of 30 kDa (Acrp30), resistin, and perilipin. Perilipin is an intracellular neutral lipid droplet protein that is hormonally regulated. This protein is localized exclusively to the surface of lipid droplets. In response to lypotic stimuli, perilipin is phosphorylated by protein kinase A. Once activated, perilipin has inhibitory affects upon hormone-sensitive lipase (HSL), a protein that mediates the hydrolysis of triacylglycerol, the major form of stored energy in the body. Perilipin expression is limited to adipocytes and steroidogenic cells. There are currently two known isoforms, Perilipin A and B. Both of these proteins are encoded by a single-copy gene and are the result of differential splicing events.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Lipid droplet-associated protein; perilipin; Perilipin-1
Gene Aliases: FPLD4; PERI; PLIN; PLIN1
UniProt ID: (Human) O60240
Entrez Gene ID: (Human) 5346
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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