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Invitrogen
BRAP Polyclonal Antibody
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Product Details
PA5-59145
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human BRAP. Recombinant protein control fragment (Product #RP-97663).
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Amount
20 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: SLVVIRLELA EHSPVPAGFG FSAAAGEMSD EEIKKTTLAS AVACLEGKSP GEKVAIIHQH LGRREMTDVI IETMKSNPDE LKTTVEERKS SEA
Highest antigen sequence identity to the following orthologs: Mouse - 84%, Rat - 83%.
Target Information
Negatively regulates MAP kinase activation by limiting the formation of Raf/MEK complexes probably by inactivation of the KSR1 scaffold protein. Also acts as a Ras responsive E3 ubiquitin ligase that, on activation of Ras, is modified by auto-polyubiquitination resulting in the release of inhibition of Raf/MEK complex formation. May also act as a cytoplasmic retention protein with a role in regulating nuclear transport.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: brap; BRAP2; BRCA1-associated protein; galectin-2-binding protein; IMP; IMP; E3 ubiquitin ligase; Impedes mitogenic signal propagation; Renal carcinoma antigen NY-REN-63; RING finger protein 52; RING-type E3 ubiquitin transferase BRAP2; unnamed protein product
Gene Aliases: 3010002G07Rik; BRAP; BRAP2; IMP; RNF52
UniProt ID: (Human) Q7Z569, (Mouse) Q99MP8
Entrez Gene ID: (Human) 8315, (Mouse) 72399, (Rat) 687346
nucleic acid binding
ubiquitin-protein transferase activity
protein binding
nuclear localization sequence binding
zinc ion binding
transferase activity
identical protein binding
metal ion binding
ubiquitin protein ligase activity
nucleotide binding
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ligase activity
ribosomal S6-glutamic acid ligase activity
ubiquitin binding
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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