Immunofluorescence analysis of 2-5-oligoadenylate synthase 3 was performed using 70% confluent log phase A549 cells treated with IFN beta 1A (1000 U/mL, O/N). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with OAS3 Polyclonal Antibody (Product # PA5-59494) at 1 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Se... View More

OAS3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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OAS3 Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using OAS3 Polyclonal Antibody (Product # PA5-59494), shows upregulation in OAS3 expression upon IFN beta 1A treatment in A549, and IFN alpha A treatment in HT-1080 and HeLa (https://doi.org/10.1073/pnas.1519657113). {TM}

Immunogen image

Immunogen Image OAS3 Polyclonal Antibody

Product Details

PA5-59494

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:1,000

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human OAS3. Recombinant protein control fragment (Product #RP-97704).

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.6 mg/mL

60 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2644932

Product Specific Information

Immunogen sequence: LKGGCDSELV IFLDCFKSYV DQRARRAEIL SEMRASLESW WQNPVPGLRL TFPEQSVPGA LQFRLTSVDL EDWMDVSLVP AFNVLGQAGS GVKPKPQV

Highest antigen sequence identity to the following orthologs: Mouse - 58%, Rat - 58%.

Target Information

OAS3 is an interferon inducible protein that belongs to the 2-5A synthetase family, may play a role in mediating resistance to virus infection, control of cell growth, differentiation, and apoptosis. OAS3 synthesizes preferentially dimeric 2',5'-oligoadenylate molecules. GTP can be an alternative substrate. OAS3 binds double-stranded RNA and polymerizes ATP into PPP(A2'P5'A)N oligomers, which activate the latent RNase L that, when activated, cleaves single-stranded RNAs. The protein is present at high level in placenta trophoblast.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: (2-5')oligo(A) synthase 3; (2-5')oligo(A) synthetase 3; 2'-5'-oligoadenylate synthase 3; 2'-5'-oligoadenylate synthetase 3, 100kDa; 2'-5'oligoadenylate synthetase p100; 2-5A synthase 3; 2-5A synthetase 3; p100 OAS; p100; interferon-induced protein; 100 kDa; p100OAS; unnamed protein product

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Gene Aliases: OAS3; P/OKcl.4; p100; p100OAS

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UniProt ID: (Human) Q9Y6K5

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Entrez Gene ID: (Human) 4940

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Function(s)

nucleotide binding 2'-5'-oligoadenylate synthetase activity RNA binding double-stranded RNA binding protein binding ATP binding transferase activity nucleotidyltransferase activity metal ion binding

Process(es)

immune system process nucleobase-containing compound metabolic process response to virus positive regulation of interferon-beta production positive regulation of tumor necrosis factor production negative regulation of chemokine (C-X-C motif) ligand 9 production RIG-I signaling pathway MDA-5 signaling pathway defense response to bacterium negative regulation of viral genome replication innate immune response defense response to virus type I interferon signaling pathway negative regulation of type I interferon-mediated signaling pathway regulation of ribonuclease activity interleukin-27-mediated signaling pathway cellular response to exogenous dsRNA positive regulation of monocyte chemotactic protein-1 production negative regulation of chemokine (C-C motif) ligand 5 production negative regulation of IP-10 production defense response to other organism antiviral innate immune response negative regulation of chemokine (C-X-C motif) ligand 2 production

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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OAS3 Polyclonal Antibody

OAS3 Antibody (PA5-59494) in ICC/IF

Product Details

PA5-59494

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Amount

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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