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Invitrogen
SPRED1 Polyclonal Antibody
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-59907
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human SPRED1. Recombinant protein control fragment (Product #RP-98123).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.2 mg/mL
Amount
20 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: GQPGLDIQSR SMEYVQRQIS KECGSLKSQN RVPLKSIRHV SFQDEDEIVR INPRDILIRR YADYRHPDMW KNDLERDDAD SSI
Highest antigen sequence identity to the following orthologs: Mouse - 94%, Rat - 94%.
Target Information
The Ras-MAP kinase pathway is essential for the differentiation of neuronal cells and myocytes; it is inhibited by Spred1, a member of the Sprouty family of proteins. Spred1 acts by suppressing the phosphorylation and activation of Raf. The Spred proteins have also been implicated in the negative feedback regulation of FGF signaling in embryogenesis and angiogenesis. Further studies have shown that expression levels of Spred1 and Spred2 proteins are inversely correlated with the incidence of tumor invasion and metastasis in human hepatocellular carcinoma (HHC), suggesting that these proteins could be useful as prognostic factors and therapeutic targets in HCC. Defects in this gene are a cause of neurofibromatosis type 1-like syndrome (NFLS).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EVH1/Sprouty domain containing protein; protein phosphatase 1, regulatory subunit 147; suppressor of Ras/MAPK activation; unnamed protein product
Gene Aliases: hSpred1; LGSS; NFLS; PPP1R147; spred-1
UniProt ID: (Human) Q7Z699
Entrez Gene ID: (Human) 161742
regulation of signal transduction
negative regulation of epithelial to mesenchymal transition
negative regulation of angiogenesis
negative regulation of transforming growth factor beta receptor signaling pathway
regulation of MAPK cascade
negative regulation of MAPK cascade
positive regulation of DNA damage response, signal transduction by p53 class mediator
vasculogenesis involved in coronary vascular morphogenesis
negative regulation of ERK1 and ERK2 cascade
negative regulation of cell migration involved in sprouting angiogenesis
negative regulation of intracellular signal transduction
negative regulation of lens fiber cell differentiation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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