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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • U2AF1 Antibodies

          Invitrogen

          U2AF1 Polyclonal Antibody

          Advanced Verification
          View all (10) U2AF1 antibodies

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          Cite U2AF1 Polyclonal Antibody

          • Antibody Testing Data (6)
          • Advanced Verification (1)
          U2AF1 Antibody in Immunocytochemistry (ICC/IF)
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          U2AF1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 7

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          U2AF1 Antibody (PA5-60843) in ICC/IF

          Immunofluorecent analysis of U2AF1 in human cell line A-431 using U2AF1 Polyclonal Antibody (Product # PA5-60843). Staining shows localization to nucleoplasm. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          U2AF1 Antibody in Immunocytochemistry (ICC/IF)
          U2AF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          U2AF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          U2AF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          U2AF1 Antibody in Immunohistochemistry (IHC)
          U2AF1 Antibody in Western Blot (WB)
          U2AF1 Antibody
          U2AF1 Polyclonal Antibody

          Product Details

          PA5-60843

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.04-0.4 µg/mL
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:1,000-1:2,500
          -

          Immunocytochemistry (ICC/IF)

          0.25-2 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant protein corresponding to Human U2AF1. Recombinant protein control fragment (Product #RP-100433).
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.2, with 40% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2649157

          Product Specific Information

          Immunogen sequence: MHLKPISREL RRELYGRRRK KHRSRS

          Highest antigen sequence identity to the following orthologs: Mouse - 100%, Rat - 100%.

          Target Information

          This gene belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: Splicing factor U2AF 35 kDa subunit; splicing factor U2AF 35kDa subunit; U2 auxiliary factor 35 kDa subunit; U2 small nuclear ribonucleoprotein auxillary factor, 35-KD subunit; U2 small nuclear RNA auxiliary factor 1; U2 small nuclear RNA auxillary factor 1; U2 snRNP auxiliary factor small subunit; U2(RNU2) small nuclear RNA auxiliary factor binding protein

          View more View less

          Gene Aliases: FP793; RN; RNU2AF1; U2AF1; U2AF35; U2AFBP

          View more View less

          UniProt ID: (Human) Q01081

          View more View less

          Entrez Gene ID: (Human) 7307

          View more View less

          Function(s)
          nucleotide binding protein binding poly(A) RNA binding metal ion binding RNA splicing factor
          Process(es)
          mRNA splicing, via spliceosome termination of RNA polymerase II transcription mRNA processing RNA export from nucleus mRNA export from nucleus RNA splicing mRNA 3'-end processing regulation of mitophagy positive regulation of protein targeting to mitochondrion
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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          We're here to help

          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

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