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Invitrogen
WAVE2 Polyclonal Antibody
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FIGURE: 1 / 15
WAVE2 Antibody (PA5-60975) in ICC/IF
Immunofluorescence analysis of WAVE2 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with WAVE2 Rabbit Polyclonal Antibody (Product # PA5-60975) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A2703... View More
Product Details
PA5-60975
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
C. elegans,
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human WAVE2. Recombinant protein control fragment (Product #RP-102976).
Conjugate
Form
Liquid
Concentration
0.20 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: DSASSPSPSF SEDNLPPPPA EFSYPVDNQR GSGLAGPKRS SVVSPSHPPP AP
Highest antigen sequence identity to the following orthologs: Mouse - 92%, Rat - 90%.
Target Information
WASP (for Wiskott-Aldrich syndrome protein) and N-WASP are downstream effectors of Cdc42 that are implicated in actin polymerization and cytoskeletal organization. The WASP family also includes VASP (vasodilator-stimulated phosphoprotein) and Mena (for mammalian enabled protein), which accumulate at focal adhesions and are also involved in the regulation of the actin cytoskeleton. The WAVE proteins are related to the WASP family proteins and are likewise involved in mediating actin reorganization downstream of the Rho family of small GTPases. The two protein homologs WAVE1 and WAVE2 specifically regulate membrane ruffling by inducing the formation of actin filament clusters in response to GTP binding and activating Rac. The WAVE proteins mediate this actin polymerization by cooperating with the Arp2/3 complex, a nucleation core, and thereby promoting the formation of actin filaments. WAVE1, which is also designated SCAR (for suppressor of cAR), is expressed primarily in the brain, while WAVE2 is widely expressed with the expression highest in peripheral blood leukocytes. WAVE3 forms a multiprotein complex that links receptor kinases and actin. Binding to actin occurs through a C-terminal verprolin homology domain in all family members. The multiprotein complex serves to tranduce signals that involve changes in cell shape, motility or function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Actin-binding protein WASF2; Protein WAVE-2; putative Wiskott-Aldrich syndrome protein family member 4; suppressor of cyclic-AMP receptor (WASP-family); Verprolin homology domain-containing protein 2; WAS protein family, member 2; WASP family protein member 2; WASP family protein member 4; WASP family Verprolin-homologous protein 2; Wiskott-Aldrich syndrome protein family member 2
Gene Aliases: dJ393P12.2; IMD2; SCAR2; WASF2; WASF4; WAVE2
UniProt ID: (Human) Q9Y6W5
Entrez Gene ID: (Human) 10163
angiogenesis
ameboidal-type cell migration
adenylate cyclase-modulating G-protein coupled receptor signaling pathway
positive regulation of lamellipodium assembly
viral process
Rac protein signal transduction
lamellipodium assembly
actin cytoskeleton organization
actin filament-based movement
megakaryocyte development
Fc-gamma receptor signaling pathway involved in phagocytosis
vascular endothelial growth factor receptor signaling pathway
negative regulation of stress fiber assembly
lamellipodium morphogenesis
cell-cell adhesion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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