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Invitrogen
RNF168 Polyclonal Antibody
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-65427
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Human RNF168. Recombinant protein control fragment (Product #RP-104814).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.2 mg/mL
Amount
20 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: LTPKSQFGSAS HSEAVQEVRK DSVSKDIDSS DRKSPTGQDT EIEDMPTLSP QISLGVGEQG ADSSIESPMP WLCACGAEWY HEGNVKTRPS NHGK
Highest antigen sequence identity to the following orthologs - mouse 43%, rat 42%.
Target Information
RNF168 was identified as a chromatin-associated RING finger protein that acts as a ubiquitin ligase both in vitro and in vivo. RNF168 targets histones H2A and H2AX, but not H2B, forming K63 polyubiquitin chains. Upon formation of DNA double strand breaks, RNF168 is recruited to the site of DNA damage where it co-localizes with -gammaH2AX and 53BP1 in an RNF8-dependent manner. This localization of RNF168 increases the local concentration of ubiquinated proteins to the threshold required for retention of the proteins 53BP1 and BRCA1, facilitating the downstream signaling cascade. Thus, RNF168 defines a new pathway demonstrating a functional cooperation between E3 ligases in genome maintenance. At least three isoforms of RNF168 are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ35794; ring finger protein 168, E3 ubiquitin protein ligase; RING-type E3 ubiquitin transferase RNF168; unnamed protein product
Gene Aliases: hRNF168; RIDL
UniProt ID: (Human) Q8IYW5
Entrez Gene ID: (Human) 165918
DNA metabolic process
double-strand break repair
double-strand break repair via nonhomologous end joining
ubiquitin-dependent protein catabolic process
cellular response to DNA damage stimulus
response to radiation
response to ionizing radiation
protein ubiquitination
negative regulation of transcription elongation from RNA polymerase II promoter
interstrand cross-link repair
regulation of gene expression, epigenetic
isotype switching
positive regulation of DNA repair
protein K63-linked ubiquitination
double-strand break repair via classical nonhomologous end joining
DNA repair-dependent chromatin remodeling
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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