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Invitrogen
POT1 Polyclonal Antibody
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FIGURE: 1 / 3
POT1 Antibody (PA5-66996) in ICC/IF
Immunofluorescence analysis of POT1 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with POT1 Polyclonal Antibody (Product # PA5-66996) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 555 (Product # A32794), (1:... View More
Product Details
PA5-66996
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Human POT1. Recombinant protein control fragment (Product #RP-105452).
Conjugate
Form
Liquid
Concentration
0.10 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: LTFEGTLGAPI IPRTSSKYFN FTTEDHKMVE ALRVWASTHM SPSWTLLKLC DVQPMQYFDL TCQLLGKAEV DGASFLLKVW DGTR
Highest antigen sequence identity to the following orthologs - mouse 76%, rat 74%.
Target Information
POT1 is a component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. It is a component of the double-stranded telomeric DNA-binding TRF1 complex which is involved in the regulation of telomere length by cis-inhibition of telomerase. Pot1 also acts as a single-stranded telomeric DNA-binding protein and thus may act as a downstream effector of the TRF1 complex and may transduce information about telomere maintenance and/or length to the telomere terminus. It binds to two or more telomeric single-stranded 5'-TTAGGG-3' repeats (G-strand) and with high specificity to a minimal telomeric single-stranded 5'-TAGGGTTAG-3' sequence. Its activity is TERT dependent but it does not increase TERT activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp586D211; hPot1; POT 1; POT-1; POT1-like telomere end-binding protein; protection of telomeres 1 homolog; Protection of telomeres protein 1
Gene Aliases: CMM10; GLM9; HPOT1; POT1
UniProt ID: (Human) Q9NUX5
Entrez Gene ID: (Human) 25913
telomere maintenance via telomerase
telomere capping
telomere assembly
positive regulation of telomere maintenance via telomerase
DNA duplex unwinding
positive regulation of helicase activity
positive regulation of telomerase activity
negative regulation of telomerase activity
positive regulation of DNA strand elongation
establishment of protein localization to telomere
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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