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Invitrogen
WEE1 Polyclonal Antibody
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FIGURE: 1 / 4
WEE1 Antibody (PA5-67019) in ICC/IF
Immunofluorescence analysis of WEE1 was performed using 70% confluent log phase HeLa treated with Geldanamycin (1 µM for 48 hours). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with WEE1 Polyclonal Antibody (Product # PA5-67019) at 2 µg/mL dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibo... View More
Product Details
PA5-67019
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Human WEE1. Recombinant protein control fragment (Product #RP-105459).
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: LLKVMIHPDPE RRPSAMALVK HSVLLSASRK SAEQLRIELN AEKFKNSLLQ KELKKAQMAK AAAEERALFT DRMATRSTTQ
Highest antigen sequence identity to the following orthologs - mouse 95%, rat 95%.
Target Information
WEE1, which is classified as a Ser/Thr kinase by sequence analysis, has been shown to inactivate multiple CDKs by tyrosine phosphorylation. Wee1 protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp686I18166; FLJ16446; WEE1 homolog; WEE1+ homolog; Wee1-like protein kinase; Wee1A kinase; WEE1hu
Gene Aliases: WEE1; WEE1A; WEE1hu
UniProt ID: (Human) P30291
Entrez Gene ID: (Human) 7465
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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