Immunofluorecent analysis of HDJ2 in human cell line U-2 OS using HDJ2 Polyclonal Antibody (Product # PA5-81929). Staining shows localization to cytosol and microtubules.

HDJ2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

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HDJ2 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with, control siRNA, HDJ2 siRNA probe #1, and HDJ2 siRNA probe #2 and a decrease in signal intensity was observed in Western Blot application using anti-HDJ2 Polyclonal Antibody (Product # PA5-81929) used at a dilution of 0.4 µg/mL. Anti-PPIB was used as a loading control. {KD}

Immunogen image

Immunogen Image HDJ2 Polyclonal Antibody

Product Details

PA5-81929

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:500

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human DNAJA1. Recombinant protein control fragment (Product #RP-95148).

3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

10 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2789090

Product Specific Information

Immunogen sequence: DQKDHAVFTR RGEDLFMCMD IQLVEALCGF QKPISTLDNR TIVITSHPGQ IVKHGDIKCV LNEGMPIYRR PYEKGRLIIE FKVNFPENGF LSPDKLSLLE KLLPERKEVE ETDEMDQVEL VDFDPNQERR RHYNGE

Highest antigen sequence indentity to the following orthologs: Mouse - 100%, Rat - 100%.

Target Information

The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1. 6 kb and a protein product with a molecular weight of about 35 kDa.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DnaJ homolog subfamily A member 1; DnaJ protein homolog 2; h HSJ-2; hDj-2; Heat shock 40 kDa protein 4; Heat shock protein J2; heat shock protein, Dnaj-like 2; HSDJ; HSJ-2; Human DnaJ protein 2; neural precursor cell expressed, developmentally down-regulated gene 7

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Gene Aliases: DNAJ2; DNAJA1; HDJ2; HSJ2; HSPF4; Nedd7

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UniProt ID: (Mouse) P63037

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Entrez Gene ID: (Mouse) 15502

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Function(s)

G-protein coupled receptor binding ATP binding Hsp70 protein binding heat shock protein binding ubiquitin protein ligase binding metal ion binding low-density lipoprotein particle receptor binding unfolded protein binding chaperone binding C3HC4-type RING finger domain binding chaperone

Process(es)

protein folding spermatogenesis response to heat sperm motility androgen receptor signaling pathway negative regulation of protein ubiquitination protein refolding DNA damage response, detection of DNA damage positive regulation of apoptotic process negative regulation of apoptotic process negative regulation of JUN kinase activity regulation of protein transport protein localization to mitochondrion toxin transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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HDJ2 Polyclonal Antibody

HDJ2 Antibody (PA5-81929) in ICC/IF

Product Details

PA5-81929

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Amount

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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