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Invitrogen
NEK9 Polyclonal Antibody
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-81941
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human NEK9. Recombinant protein control fragment (Product #RP-89014).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.1 mg/mL
Amount
10 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: VSCGDEFTIA ATDDNHIFAW GNGGNGRLAM TPTERPHGSD ICTSWPRPIF GSLHHVPDLS CRGWHTILIV EKVLNSKTIR SNSSGLSIGT VFQSSSPGGG GGGGGGEEED SQQESETPDP SGGFRGTMEA DRGMEGLISP TEAM
Highest antigen sequence indentity to the following orthologs: Rat - 97%, Mouse - 94%.
Target Information
NEK9, a NEK type protein kinase, regulates chromosome alignment and segregation in mitosis. The protein has a N-terminal NIMA-like catalytic domain, a central domain with homology to the guanine nucleotide exchange factor for the GTPase Ran (RCC1), and a C-terminal coiled-coil domain. It is phosphorylated by active p34(Cdc2) and is capable of autophosphorylation and oligomerization. The activated protein binds Nek6 and RCC1, and has been shown to associate with the human homolog of the Drosophila protein Bicaudal D (Bicd2) in vivo and to phosphorylate this coiled-coil protein in vitro. NEK9 expression has been documented in normal human brain. ESTs have been isolated from several tissue libraries, including normal bone, lymph and thymus and cancerous cervix, eye, lymph and placenta.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp434D0935; MGC138306; MGC16714; NEK 9; Nek8; Nercc 1 kinase; Nercc1 kinase; Never in mitosis A-related kinase 9; NimA-related kinase 8; NimA-related protein kinase 9; Serine/threonine-protein kinase Nek9
Gene Aliases: KIAA1995; NEK8; NEK9; NERCC
Entrez Gene ID: (Human) 91754
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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