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Invitrogen
P4HTM Polyclonal Antibody
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FIGURE: 1 / 5
P4HTM Antibody (PA5-82286) in IHC (P)
Immunohistochemical analysis of P4HTM in human pancreas using P4HTM Polyclonal Antibody (Product # PA5-82286) shows strong cytoplasmic/membranous positivity in exocrine glandular cells and moderate cytoplasmic/membranous positivity in endocrine glandular cells.
Product Details
PA5-82286
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human P4HTM. Recombinant protein control fragment (Product #RP-89701).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.1 mg/mL
Amount
10 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: KADPDGDGVL SLQEFSNMDL RDFHKYMRSH KAESSELVRN SHHTWLYQGE GAHHIMRAIR QRVLRLTRLS PEIVELSEPL QVVRYGEGGH YHAHVDSGPV YPETICSHTK LVANESVPFE TSCRQVSPNW GLPSILRPGT P
Highest antigen sequence indentity to the following orthologs: Mouse - 85%, Rat - 86%.
Target Information
Prolyl hydroxylase 4 is a prolyl hydroxylase that modifies HIF-alpha. Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the prolyl hydroxylase sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. Prolyl hydroxylase is an essential component of the pathway through which cells sense oxygen. In the presence of oxygen, prolyl hydroxylases convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at the cellular level, cause the HIF conversion to be reduced so that HIF is stable and there is increased angiogenesis. Prolyl hydroxylase 4, specifically, catalyzes the posttranslational formation of 4-hydroxyproline in HIF alpha proteins. It may function as a cellular oxygen sensor and, under normoxic conditions, may targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ20262; HIF-PH4; HIF-prolyl hydroxylase 4; HPH-4; hypoxia-inducible factor prolyl 4-hydroxylase; Hypoxia-inducible factor prolyl hydroxylase 4; P4H TM; P4H with transmembrane domain; P4H-TM; PH 4; proline 4-hydroxylase; prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum); Prolyl hydroxlase domain-containing 4; Transmembrane prolyl 4-hydroxylase; unnamed protein product
Gene Aliases: EGLN4; HIDEA; HIFPH4; P4H-TM; P4HTM; PH-4; PH4; PHD4
UniProt ID: (Human) Q9NXG6
Entrez Gene ID: (Human) 54681
procollagen-proline 4-dioxygenase activity
iron ion binding
calcium ion binding
oxidoreductase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
L-ascorbic acid binding
metal ion binding
dioxygenase activity
hypoxia-inducible factor-proline dioxygenase activity
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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