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Invitrogen
APAF1 Polyclonal Antibody
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Product Details
PA5-83190
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human APAF1. Recombinant protein control fragment (Product #RP-95285).
Conjugate
Form
Liquid
Concentration
0.05 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: PLVVSLIGAL LRDFPNRWEY YLKQLQNKQF KRIRKSSSYD YEALDEAMSI SVEMLREDIK DYYTDLSILQ KDVKVPTKVL CILWDMETEE VEDILQEFVN KSLLFCDR
Highest antigen sequence indentity to the following orthologs: Mouse - 95%, Rat - 94%.
Target Information
APAF1 gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: APAF-1; Apaf-1XL; Apaf1 variant; Apoptotic protease-activating factor 1; cytochrome c and dATP-dependent procaspase-9 activation factor; DKFZp781B1145; KIAA0413; similar to C. elegans cell death gene ced-4
Gene Aliases: APAF-1; APAF1; CED4; KIAA0413
UniProt ID: (Human) O14727
Entrez Gene ID: (Human) 317
response to hypoxia
kidney development
neural tube closure
apoptotic process
nervous system development
brain development
response to nutrient
cardiac muscle cell apoptotic process
cell differentiation
forebrain development
regulation of apoptotic process
positive regulation of apoptotic process
system development
neuron apoptotic process
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
cellular response to transforming growth factor beta stimulus
response to G1 DNA damage checkpoint signaling
apoptotic signaling pathway
intrinsic apoptotic signaling pathway
regulation of apoptotic DNA fragmentation
positive regulation of apoptotic signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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