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Invitrogen
DNA-PK Polyclonal Antibody
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Product Details
PA5-83261
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human PRKDC. Recombinant protein control fragment (Product #RP-97237).
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
50 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: ETLSLMTKEI SSVPCWQFIS WISHMVALLD KDQAVAVQHS VEEITDNYPQ AIVYPFIISS ESYSFKDTST GHKNKEFVAR IK
Highest antigen sequence indentity to the following orthologs: Mouse - 80%, Rat - 76%.
Target Information
DNA-PK is a heterotrimeric, DNA-dependent protein kinase that initiates signaling cascades in response to DNA damage. DNA-PK is an atypical kinase, closely related to the PI3 kinases, and is often potently inhibited by small molecules designed to target PI3 kinases. DNA-PK encodes a component of the mediator complex, a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. Multiple transcript variants encoding different isoforms have been found for DNA-PK.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA-activated protein kinase catalytic subunit; DNA-dependent protein kinase catalytic subunit; DNA-PK catalytic subunit; DNPK1; hyper-radiosensitivity of murine scid mutation, complementing 1; p460; protein kinase, DNA-activated, catalytic polypeptide; S473K; Ser-473 kinase; unnamed protein product
Gene Aliases: DNA-PKC; DNA-PKcs; DNAPK; DNAPKc; DNPK1; HYRC; HYRC1; IMD26; p350; PRKDC; S473K; XRCC7
UniProt ID: (Human) P78527
Entrez Gene ID: (Human) 5591
nucleotide binding
DNA binding
double-stranded DNA binding
RNA binding
protein kinase activity
protein serine/threonine kinase activity
DNA-dependent protein kinase activity
protein binding
ATP binding
kinase activity
transferase activity
enzyme binding
protein domain specific binding
U3 snoRNA binding
histone H2AXS139 kinase activity
RNA polymerase II sequence-specific DNA binding transcription factor binding
protein serine kinase activity
maturation of 5.8S rRNA
telomere maintenance
double-strand break repair via homologous recombination
somitogenesis
negative regulation of protein phosphorylation
activation of innate immune response
B cell lineage commitment
immature B cell differentiation
pro-B cell differentiation
T cell lineage commitment
immune system process
DNA repair
double-strand break repair
double-strand break repair via nonhomologous end joining
DNA recombination
protein phosphorylation
cellular response to DNA damage stimulus
brain development
heart development
intrinsic apoptotic signaling pathway in response to DNA damage
response to ionizing radiation
response to gamma radiation
telomere capping
peptidyl-serine phosphorylation
peptidyl-threonine phosphorylation
lymphocyte differentiation
replication fork processing
mitotic G1 DNA damage checkpoint
protein destabilization
cellular response to insulin stimulus
T cell differentiation in thymus
V(D)J recombination
immunoglobulin V(D)J recombination
T cell receptor V(D)J recombination
small-subunit processome assembly
ectopic germ cell programmed cell death
protein modification process
ribosome biogenesis
regulation of circadian rhythm
positive regulation of apoptotic process
negative regulation of apoptotic process
innate immune response
positive regulation of lymphocyte differentiation
positive regulation of erythrocyte differentiation
positive regulation of translation
positive regulation of transcription from RNA polymerase II promoter
rhythmic process
regulation of smooth muscle cell proliferation
regulation of epithelial cell proliferation
protein localization to chromatin
regulation of cellular response to stress
double-strand break repair via alternative nonhomologous end joining
DNA repair-dependent chromatin remodeling
cGAS/STING signaling pathway
negative regulation of cGAS/STING signaling pathway
regulation of hematopoietic stem cell differentiation
positive regulation of platelet formation
positive regulation of double-strand break repair via nonhomologous end joining
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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