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Invitrogen
PIK3CB Polyclonal Antibody
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FIGURE: 1 / 6
PIK3CB Antibody (PA5-84631) in ICC/IF
Immunofluorescence analysis of Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform was performed using 70% confluent log phase T-47D and MDA-MB-231 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with PIK3CB Polyclonal Antibody (Product # PA5-84631) at 0.5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG ... View More
Product Details
PA5-84631
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human PIK3CB. Recombinant protein control fragment (Product #RP-105302).
Conjugate
Form
Liquid
Concentration
0.3 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: LSLVGLSWMD WLKQTYPPEH EPSIPENLED KLYGGKLIVA VHFENCQDVF SFQVSPNMNP IKVNELAIQK RLTIHGK
Highest antigen sequence indentity to the following orthologs: Mouse - 87%, Rat - 87%.
Target Information
PIK3CB (phosphatyidylinositol 4,5-biphosphate 3-kinase catalyic subunit beta isoform) is a phosphoinositide-3-kinase that phosphorylates PtdIns (phosphatidylinositol), PtdIns4P (phosphatidylinositol 4-phosphate), and PtdIns(4,5)P2 (phosphatidylinositol 4,5-biphosphate) to generate phosphatidylinositol 3,4,5-triphosphate (PIP3). PIP3 plays a key role in recruiting PH domain-containing proteins to the membrane and activating signaling cascades involved in cell growth, survival, proliferation, motility, and morphology.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 110 kda catalytic subunit; Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit beta; Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform; phosphoinositide-3-kinase, catalytic, beta polypeptide; PI3-kinase p110 subunit beta; PI3-kinase subunit beta; PI3K-beta; pi3kcb; PtdIns-3-kinase p110; ptdIns-3-kinase subunit beta; PtdIns-3-kinase subunit p110-beta; Serine/threonine protein kinase PIK3CB; unnamed protein product
Gene Aliases: P110BETA; PI3K; PI3KBETA; PIK3C1; PIK3CB
UniProt ID: (Human) P42338
Entrez Gene ID: (Human) 5291
nucleotide binding
protein binding
ATP binding
kinase activity
1-phosphatidylinositol-3-kinase activity
transferase activity
1-phosphatidylinositol-4-phosphate 3-kinase activity
insulin receptor substrate binding
phosphatidylinositol-4,5-bisphosphate 3-kinase activity
phosphatidylinositol kinase activity
protein serine kinase activity
endothelial cell proliferation
regulation of cell-matrix adhesion
leukocyte mediated immunity
response to ischemia
sphingosine-1-phosphate signaling pathway
lipid metabolic process
cellular calcium ion homeostasis
endocytosis
autophagy
chemotaxis
cell adhesion
homophilic cell adhesion via plasma membrane adhesion molecules
signal transduction
transmembrane receptor protein tyrosine kinase signaling pathway
G-protein coupled receptor signaling pathway
positive regulation of autophagy
positive regulation of endothelial cell migration
positive regulation of gene expression
cell migration
platelet activation
positive regulation of neutrophil apoptotic process
positive regulation of Rac protein signal transduction
phosphatidylinositol-3-phosphate biosynthetic process
embryonic cleavage
natural killer cell mediated cytotoxicity
negative regulation of MAPK cascade
protein kinase B signaling
innate immune response
positive regulation of nitric oxide biosynthetic process
phosphatidylinositol phosphorylation
phosphatidylinositol-mediated signaling
negative regulation of protein kinase B signaling
angiogenesis involved in wound healing
platelet aggregation
negative regulation of vascular endothelial growth factor signaling pathway
negative regulation of hypoxia-induced intrinsic apoptotic signaling pathway
negative regulation of sprouting angiogenesis
regulation of clathrin-mediated endocytosis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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