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Invitrogen
GOLGB1 Polyclonal Antibody
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FIGURE: 1 / 2
GOLGB1 Antibody (PA5-100542) in ICC/IF
Immunocytochemistry analysis of GOLGB1 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with GOLGB1 polyclonal antibody (Product # PA5-100542) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-100542
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human GOLGB1(Accession Q14789), corresponding to amino acid residues E1787-N1837.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total Giantin.
Target Information
Golgin subfamily B member 1 (GOLGB1) is a single pass type I membrane protein located in the golgi apparatus membrane. GOLGB1 may participate in forming intercisternal cross-bridges of the Golgi complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 372 kDa Golgi complex-associated protein; giantin; golgi autoantigen, golgin subfamily b, macrogolgin (with transmembrane signal), 1; golgi integral membrane protein 1; golgin B1, golgi integral membrane protein; unnamed protein product
Gene Aliases: GCP; GCP372; GOLIM1
UniProt ID: (Human) Q14789
Entrez Gene ID: (Human) 2804
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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