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Invitrogen
NPLOC4 Polyclonal Antibody
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FIGURE: 1 / 2
NPLOC4 Antibody (PA5-100647) in ICC/IF
Immunocytochemistry analysis of NPLOC4 in HepG2 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with NPLOC4 polyclonal antibody (Product # PA5-100647) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-100647
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human NPLOC4(Accession Q8TAT6), corresponding to amino acid residues G173-V223.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total NPLOC4.
Target Information
NPLOC4 gene ontology annotations related to this gene include ubiquitin-dependent protein catabolic process.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ20657; homolog of yeast nuclear protein localization 4; NPLOC4 ubiquitin recognition factor; nuclear protein localization 4 homolog; unnamed protein product
Gene Aliases: mKIAA1499; NPL4
UniProt ID: (Human) Q8TAT6, (Rat) Q9ES54, (Mouse) P60670
Entrez Gene ID: (Human) 55666, (Rat) 140639, (Mouse) 217365
ubiquitin-dependent protein catabolic process
Golgi organization
retrograde protein transport, ER to cytosol
negative regulation of type I interferon production
ERAD pathway
negative regulation of RIG-I signaling pathway
proteasome-mediated ubiquitin-dependent protein catabolic process
rescue of stalled ribosome
ribosome-associated ubiquitin-dependent protein catabolic process
membrane fusion
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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